Taro is cultivated in most Regions of Cameroon and it is affected by taro leaf blight disease since 2010 which has decreased its production. Lack of disease-free planting materials has been a main problem to farmers. This study was carried out at International Institute of Tropical Agriculture (IITA) Yaounde and Institute of Agricultural Research for Development (IRAD) Bambui to assess different substrates for acclimatization of tissue culture taro plantlets in apropagator. No information is available on acclimatization of Cameroonian taro plantlets in different substrates. Taro plantlets from tissue culture were acclimatised in a propagator for six weeks under different substrates, the first substrate consisted of sterile three parts of soil and one part of river sand mixed together (3:1), the second substrate consisted of sterile two parts of soil and two parts of river sand mixed together (2:2), the third substrate consisted of sterile two parts of soil, one part of rice husk and one part of river sand mixed together (2:1:1) and the fourth substrate consisted of sterile one part of soil and three parts of river sand mixed together (1:3). After acclimatisation of the different taroplantlets (Dark green petiole with small leaves (L1), Red petiole with small leaves (L2), Light green petiole with large leaves (L3) and Light green petiole with small leaves (L4) in these four substrates, it was observed that the best growth rate of plant was recorded on substrate sand + soil (1:3). The other substrates showed moderate growth of plants. Substrate sand + soil (1:3) can be recommended for acclimatization of Cameroonian taro plantlets.
References
[1]
Rao, R., Hunter, D., Eyzaguirre, P. and Mathews, P. (2010) Ethnobotany and Global Diversity Taro. In: Rao, V., Mathews, P.J., Ezyaguire, P.B. and Hunter, D., Eds., The Global Diversity of Taro: Ethnobotany and Conservation, Ramanatha Bioversity International, Rome, 2-5.
[2]
Rochani, A. (1994) The Role of Taro (Colocasia esculenta) in Livelihood of Local People in the Ayanna Sub-District Sorong Iran Jaya Province. In: Jackson, G. and Wagah, M.E., Eds., The Second Taro Symposium, Manokwari, Indonesia, 105-109.
[3]
Deo, P.C., Tyagi, A.P., Taylor, M., Becker, D.K. and Harding, R.M. (2009) Improving Taro (Colocasia esculenta var. esculenta) Production Using Biotechnological Approaches. South Pacific Journal of Natural Science, 27, 6-13. https://doi.org/10.1071/SP09002
[4]
Fokunang, C.N., Mbong, G., Manju, E.B., Tembe, E. and Rachid, H. (2016) Screen House and Field Resistance of taro Cultivars to Taro Leaf Blight Disease (Phytopthoracolocasiae). British Biotechnology Journal, 15, 1-15. https://doi.org/10.9734/BBJ/2016/14317
[5]
Onwueme, I. (1999) Taro Cultivation in Asia and the Pacific. Food and Agriculture Organization of the United Nations Regional office for Asia and the Pacific. Bangkok, 15.
[6]
Misra, R.S. and Sriam, S. (2002) Medicinal Value and Export Potential of Tropical Tuber Crops. In: Govili, J.N., Pandey, J., Shivkumar, B.G. and Singh, V.K., Eds., Series Recent Progress in Medicinal Plants Crop Improvement, Production Technology and Commerce, 376-386.
[7]
Misra, R.S., Sahu, A.K., Maheswari, S.K., Sriram, S., Sharma, K. and Suhu, A.K. (2007) Integratedmanagement of Phytophthora Leaf Blight Disease of Taro (Colocasiaeesculanta (L.) Schott). Journal of Root Crops, 33, 144-146.
[8]
Boampong, R., Aboagye, L.M., Nyadanu, D., Essilfie, M.E. and Adu Amoah, R. (2019) Biochemical Characterization of Some Taro (Colocasia esculenta L. Schott) Germplasm in Ghana. Advances in Agriculture, 2019, Article ID: 1965761. https://doi.org/10.1155/2019/1965761
[9]
Ramanatha, R.V., Matthews, P.J., Eyzaguirre, P.B. and Hunter, D. (2010) The Global Diversity of Taro: Ethnobotany Coservation. Biodiversity International, Rome, 202.
[10]
Vinning, G. (2003) Select Market for Taro, Sweet Potato and Yam: A Report for the Rural Industries Research and Development Corporation. RIRDC Publication No.03/052 Kingston, ACT. http://ctalogue.nla.gov.au/Record/997163
[11]
Li, Y., XU, C. and Chen, J. (2002) Establishment of Virus-Free Taro (Colocasia esulenta cv. Fenghuayunaitou) by Meristem-Tip Culture Combined with Thermotherapy. Plant Pathology Journal, 1, 40-43. https://doi.org/10.3923/ppj.2002.40.43
[12]
Manner, H.I. and Taylor, M. (2010) Farm and Forestry Production and Marketing Profile for Taro (Colocasia esculenta). In: Elevitch, C.R., Ed., Specialty Crops for Pacific Island Agroforestry, Permanent Agriculture Resources (PAR), Holualoa, 1-34.
[13]
Mbong, G.A., Fokunang, C.N., Lum, A.F., Bambot, M.B. and Tembe, E.A. (2013) An Overview of Phytophthora colocasiae of Cocoyams: A Potential Economic Disease of Food Security in Cameroon. Discourse Journal of Agriculture and Food Sciences, 1,140-145.
[14]
Manju, E.B., Fokunang, C.N., Mbong, G.A., Tima, T.S., Suh, C., Tembe-Fokunang, E.A. and Hanna, R. (2017) Impact of Fungicide Application on Taro Leaf Blight Disease in Three Regions Cameroon. Journal of Experimental Agriculture International, 17, 1-23. https://doi.org/10.9734/JEAI/2017/33915
[15]
Omane, E., Oduro, K.A., Cornelius, E.W., Opuku, L.Y., Akrofi, A.Y., Sharma, K., Lava Kumar, S. and Bandyopadhyay, R. (2012) First Report of Leaf Blight of Taro (Colocasia esculenta) Caused by Phtophthora colocasiae in China. Plant Diseases, 96, 292. https://doi.org/10.1094/PDIS-09-11-0789
[16]
FAO (2020) Food and Agriculture Organization of United Nations. http://www.tridge.com
[17]
Abo El-Nil, M.M. and Zettler, F.W. (1976) Callus Initiation and Organ Differentiation from Shoot Tip Cultures of Colocasia esculenta. Plant Science Letters, 6, 401-408. https://doi.org/10.1016/0304-4211(76)90122-X
[18]
Behera, K.K. and Sahoo, S. (2008) In Vitro Micro Propagation of Colocasia esculenta (L.) Schott. (CV Local-Jhankhri) through Corm Sprouts. The Orissa Journal of Horticulture, 36, 50-54.
[19]
Purmadewi, G.C., Wulandari, A.S. and Damayanti, R.U. (2019) The Effect of Rooting and Acclimatization Media on the Success of Acclimatization of Tembesu (Fagraea fragrans (Roxb.) Miq.). IOP Conference Series: Earth and Environmental Science, 7, Article ID: 032023.
[20]
Manju, E., Mbong, G., Fokunang, C., Tembe-Fokunang, E. and Rachid, H. (2017) Application of in-Vitro Micro Propagation Technique for Sustainable Production of Four Local Taro Cultivars (Colocasia esculent (L) Schott). Africa Journal of Biotechnology, 16, 1638-1645. https://doi.org/10.5897/AJB2017.15921
[21]
Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiology of Plant, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
[22]
Hartmann, K.D. (1990) Plant Propagation—Principles and Practices. Regents/Prentice Hall Press, Hoboken, 647.
[23]
Heanes, D.L. (1984) Determination of Total Organic-C in Soils by an Improved Chromic Acid Digestion and Spectrophotometric Procedure. Communications in Soil Science and Plant Analysis, 15, 1191-1213. https://doi.org/10.1080/00103628409367551
[24]
Buondonno, A., Rashad, A.A. and Coppola, E. (1995) Comparing Tests for Soil Fertility. The Hydrogen Peroxide/Sulphuric Acid Treatment as an Alternative to the Copper/Selenium Catalyzed Digestion Process for Routine Determination of Soil Nitrogen-Kjeldahl. Communication in Soil Science and Plant Analysis, 26, 1607-1619. https://doi.org/10.1080/00103629509369394
[25]
Anderson, J.M. and Ingram, J.S.I. (1993) Tropical Soil Biology and Fertility: A Handbook of Methods. Soil Science, 157, 265.
[26]
Mehlich, M. (1984) Mehlich 3 Soil Test Extractant: A Modification of the Mehlich 2 Extractant. Communications in Soil Science and Plant Analysis, 15, 1409-1416. https://doi.org/10.1080/00103628409367568
[27]
Murphy, J. and Riley, P. (1962) A Modified Single Solution Method for Determination of Phosphate in Natural Waters. Analytica Chimica Acta, 27, 31-36. https://doi.org/10.1016/S0003-2670(00)88444-5
[28]
Jr Benton, J. and Vernon, W.C. (1990) Sampling, Handling and Analyzing Plant Tissue Samples. In: Westerman, R.L., Ed., Soil Testing and Plant Analysis, SSSA Book Series No.3, Soil Science Society of America, Madison, 389-427.
[29]
Riaz, A., Arshad, M., Younis, A., Atiq, R. and Mansoor, H. (2008) Effects of Different Growing Mediaon Growth and Flowering of Zinnia Elegaris CV. Blue Point. Pakistan Journal of Botany, 40, 1579-1585.
[30]
Dewayne, L.I., Richard, W.H. and Thomas, H.Y. (2003) Growth Media for Container Grown Ornamel Plants. The Environmental Horticulture Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences University of Florida, BUL 24. 1-6.
[31]
Legari, S.J., Wahocho, N.A., Laghari, G.M., HafeezLaghari, A., MustafaBhabhan, G., HussainTalpur, K. and Lashari, A.A. (2016) Role of Nitrogen for Plant Growth and Development: A Review. Advances in Environmental Biology, 10, 209-219.
[32]
Ahmad, S.R., Ahmad, M.Y., Ashraf, M. and Waraich, E.A. (2009) Sunflower (Helianthus annuus L.) Responses to Drought Stress at Germination and Seedling Growth Stages. Pakistan Journal of Botany, 41, 647-654.
[33]
Ali, A., Choudhry, M.A., Malik, M.A., Ahmad, R. and Saifullah. (2000) Effect of Various Doses of Nitrogen on the Growth and Yield of Two Wheat Cultivar. Pakistan Journal of Biological Science, 3, 1004-1005. https://doi.org/10.3923/pjbs.2000.1004.1005
[34]
Shah, J.L., Niaz, A.W., Ghulam, M.L., Abdul, H.L., Ghulam, M.B., Khalid, H.T., Figureue, A.B., Safdar, A.W. and Ayaz, A.L. (2016) Role of Nitrogen for Plant Growth and Development. Journal Advances in Environmental Biology, 10, 209-218.
[35]
Philip, J.W. and Martin, R.B. (2003) Calcium in the Plant. Annals of Botany, 92, 487-511. https://doi.org/10.1093/aob/mcg164
[36]
Rout, G.R. and Sahoo, S. (2015) Role of Iron in Plant Growth and Metabolism. Review in Agricultural Science, 3, 1-24. https://doi.org/10.7831/ras.3.1
[37]
Chung, R.C. and Goh, C.J. (1994) High Frequency Direct Shoot Regeneration from Corm Axillary Buds Andrapid Clonal Propagation of Taro, Colocasia esculenta var. Esculenta (L.) Schott (Araceae). Plant Science, 104, 93-100. https://doi.org/10.1016/0168-9452(94)90194-5
[38]
Erisma, J.W., Sutton, M.A., Galloway, J., Klimon, Z. and Winiwarter, W. (2008) How a Century of Ammonia Synthesis Changed the World. Nature Geoscience, 1, 636-639. https://doi.org/10.1038/ngeo325
[39]
Hazarika, B.N. (2003) Acclimatization of Tissue-Cultured Plants. Current Science, 85, 428-438.
