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Botanical Research 2023
拟南芥尿黑酸双加氧酶基因突变体转录组分析
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Abstract:
尿黑酸双加氧酶(Homogentisate dioxygenase, HGO)催化酪氨酸降解代谢第三步反应。HGO突变会促进拟南芥生长。该研究对拟南芥hgo突变体和野生型的转录组进行分析，探究HGO影响拟南芥生长的机制。结果表明，突变体和野生型间1413个基因表达存在差异；其中741个基因在突变体中表达上调，672个基因在突变体中表达下调。GO功能注释显示，差异基因显著富集在生长素响应、氨基酸代谢、谷胱甘肽代谢等过程。KEGG通路分析显示，差异基因主要涉及苯丙烷类生物合成、类黄酮生物合成、MAPK信号途径、谷胱甘肽代谢、植物激素信号传导、淀粉和蔗糖代谢等通路。研究结果表明HGO可能通过影响苯丙烷类生物合成和激素信号传导等调控拟南芥生长。该研究结果可为进一步探究酪氨酸降解代谢在植物中的功能奠定基础。
Homogentisate dioxygenase (HGO) catalyzes the third step of tyrosine catabolism. HGO mutation promoted growth of Arabidopsis. Transcriptome sequencing of the hgo mutant and wild type was performed to elucidate the mechanism of HGO function. The results were as follows: 1413 differentially expressed genes were identified between the mutant and wild type. Among them, 741 genes were up-regulated, and 672 genes were down-regulated in the mutant. GO annotation analysis indicated that these genes were significantly enriched in auxin response, amino acid metabolism, and glutathione metabolism. KEGG revealed that differentially expressed genes mainly participated in phenylpropanoid biosynthesis, flavonoid biosynthesis, MAPK signaling pathway, glutathione metabolism, plant hormone signal transduction, starch and sucrose metabolism. The data suggested that HGO might regulate Arabidopsis development by modulating phenylpropanoid biosynthesis and plant hormone signal transduction, providing a basis for exploring function of tyrosine catabolism in plants.
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