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柚皮素对视网膜缺血/再灌注损伤大鼠HO-1和Caspase-3的影响
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Abstract:
目的：观察柚皮素处理后视网膜缺血再灌注损伤(retinal ischemia-reperfusion injury, RIRI)大鼠视网膜血红素氧合酶-1 (hemeoxygenase-1, HO-1)和caspase-3的变化，探讨柚皮素治疗RIRI的作用机制，为视网膜氧化应激的治疗提供新的的治疗方案。方法：取55只Wistar250-33g健康大鼠随机分为正常对照组5只，RIRI组25只，NAR + RIRII组25只(柚皮素组腹腔注射100 mg/kg柚皮素，每天1次，连续7 d后采用高眼压法制成RIRI模型，后两组于RIRI后12 h、24 h、48 h、3 d、7 d各处死5只鼠。采用尼氏染色观察HPC对各组大鼠视网膜的影响，免疫组织化学法动态观察柚皮素对RIRI大鼠HO-1、caspase-3表达的影响。结果：尼氏染色显示正常组视网膜结构清晰，RIRI组大鼠视网膜各层水肿，分布紊乱、细胞稀疏，柚皮素处理组与RIRI组比较，各层组织水肿明显减轻，细胞排列较规整。正常大鼠视网膜组织HO-1、caspase-3极少量表达，RIRI后RIRI组HO-1表达升高，NAR + RIRII组HO-1表达较RIRI组升高，RIRI组caspase-3表达升高，NAR + RIRII组caspase-3表达较RIRI组减少；与RIRI组比较，NAR + RIRII组HO-1阳性细胞数增多，caspase-3蛋白表达减少，差异有统计学意义(P < 0.05)。结论：柚皮素可促进缺血再灌注损伤大鼠视网膜HO-1蛋白，减少caspase-3的表达，减轻视网膜损伤，具有视网膜保护作用。
Objective: To observe the dynamic changes of retinal heme oxygenase-1 and caspase-3 in rats with retinal ischemia-reperfusion injury( RIRI) after naringin treatment, and to explore the mechanism of naringin in the treatment of RIRI, so as to provide a new treatment scheme for RIRI. Methods: Fifty-five Wistar adult healthy rats were randomly divided into normal control group (N = 5), RIRI group (RIRI = 25) and NAR + RIRII group (NAR + RIRI = 25) (naringin group was intraperitoneally injected with 100 mg/kg naringin once a day for 7 days, and the RIRI model was established by intraocular pressure method, Five rats in the latter two groups were sacrificed at 12 h, 24 h, 48 h, 3 d and 7 d after RIRI. The effects of HPC on retina of RIRI rats were observed by Nissl staining, and the effects of naringin on the expression of HO-1 and caspase-3 in RIRI rats were dynamically observed by immunohistochemistry. Results: Nissl’s staining showed that the retina structure of rats in the normal group was clear, while that of rats in the RIRI group was highly edema, with disordered and sparse distribution. Compared with the RIRI group, the edema of cells in each layer was significantly reduced in naringin treatment group, and the cell arrangement was more orderly. The expression of HO-1 and caspase-3 in retina tissue of normal rats was very small. After RIRI, the expression of HO-1 in RIRI group was increased, the expression of Ho-1 in NAR + RIRII group was higher than that in RIRI group, and the expression of caspase-3 in RIRI group was increased. The expression of caspase-3 in NAR + RIRII group was lower than that in RIRI group. Compared with RIRI group, the number of Ho-1 positive cells in NAR + RIRII group increased, and the expression of caspase-3 protein decreased, with statistical significance (P < 0.05).Conclusion: Naringin can promote the retinal Ho-1 protein,
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