Alteration in skin microbiome profile is involved in many skin conditions, therefore, microbiome modulation is a reasonable target for skin health. Probiotic skincare was suggested, and leave-on synbiotic skincare preparation, blending bacillus spores, as probiotic active ingredient, and inulin sugar, as prebiotic booster, is evaluated, using microarray, 16S gene sequencing, and clinical skin analyses. Topical application of leave-on synbiotic skincare on skin model activates a profound effect on skin biology, expressed in the transcriptome level, with higher than 10% affected genes. The significance of the synbiotic preparation on skin biology was assured, indicating the involvement of major biological pathways. Blending probiotic with Polygonum aviculare plant extract, had triggered a distinct gene expression alteration, demonstrating the sensitivity of skin responses to different active substances. A synbiotic skincare application, had successfully introduced live and active Bacillus probiotics on human skin, detectable days after application was finalized. Following synbiotic application, the microbial content of several microorganisms, related to skin pathologies, was reduced, while the content of bacillus species, representing a healthier microbiome status, was increased, suggesting that frequent application may lead to overall healthier skin microbiome. Acne vulgaris involves unbalanced skin microbiome, with expansion of Cutibacterium acnes. The efficacy of a synbiotic skin cream was assessed to improve acne symptoms, including IGA dermatologist acne severity score, the number of counted acne lesions, measured skin oiliness and skin redness, visual appearance of skin-pores, skin smoothness and softness, and Acne Quality of Life Index.
References
[1]
Aggarwal, N., Kitano, S., Puah, G.R.Y., Kittelmann, S., Hwang, I.Y. and Chang, M.W. (2023) Microbiome and Human Health: Current Understanding, Engineering, and Enabling Technologies. Chemical Reviews, 123, 31-72. https://doi.org/10.1021/acs.chemrev.2c00431
[2]
Zheng, D.P., Liwinski, T. and Elinav, E. (2020) Interaction between Microbiota and Immunity in Health and Disease. Cell Research, 30, 492-506. https://doi.org/10.1038/s41422-020-0332-7
[3]
Hou, K.J., Wu, Z.X., Chen, X.Y., Wang, J.Q., Zhang, D.Y., Xiao, C.X., Zhu, D., Koya, J.B., Wei, L., Li, J.L. and Chen, Z.S. (2022) Microbiota In Health and Diseases. Signal Transduction and Targeted Therapy, 7, Article No. 135. https://doi.org/10.1038/s41392-022-00974-4
[4]
Pistone, D., Meroni, G., Panelli, S., D’Auria, E., Acunzo, M., Pasala, A.R., Zuccotti, G.V., Bandi, C. and Drago, L. (2021) A Journey on the Skin Microbiome: Pitfalls and Opportunities. International Journal of Molecular Sciences, 22, Article 9846. https://doi.org/10.3390/ijms22189846
[5]
Yang, Y., Qu, L., Mijakovic, I., et al. (2022) Advances in the Human Skin Microbiota and Its Roles in Cutaneous Diseases. Microbial Cell Factories, 21, Article No. 176. https://doi.org/10.1186/s12934-022-01901-6
[6]
Boxberger, M., Cenizo, V., Cassir, N. and La Scola, B. (2021) Challenges in Exploring and Manipulating the Human Skin Microbiome. Microbiome, 9, Article No. 125. https://doi.org/10.1186/s40168-021-01062-5
[7]
Yu, J.Y., Ma, X.M., Wang, X.Y., Cui, X.T., Ding, K., Wang, S.Y. and Han, C.C. (2022) Application and Mechanism of Probiotics in Skin Care: A Review. Journal of Cosmetic Dermatology, 21, 886-894. https://doi.org/10.1111/jocd.14734
[8]
Chen, Y.E., Fischbach, M.A. and Belkaid, Y. (2018) Skin Microbiota-Host Interactions. Nature, 553, 427-436. https://doi.org/10.1038/nature25177
[9]
Mintel—Google Trends in 2021 (2022)—A Special Report—Monitoring New Skincare Products and Innovation.
[10]
Coates, H. (2023) 10 Skin-Care Trends That Will Dominate 2023. VOGUE, 2 January 2023.
[11]
Temmerman, R. and Meersman, F. (2022) Synbiotic Skin Care with Bacillus Spores. Personal Care Magazine.
[12]
Klassert, T.E., Zubiria-Barrera, C., Neubert, R., Stock, M., Schneegans, A., López, M., Driesch, D., Zakonsky, G., Gastmeier, P., Slevogt, H. and Leistner, R. (2022) Comparative Analysis of Surface Sanitization Protocols on the Bacterial Community Structures in the Hospital Environment. Clinical Microbiology and Infection, 28, 1105-1112. https://doi.org/10.1016/j.cmi.2022.02.032
[13]
Seo, S.H., Lee, S.H., Cha, P.H., Kim, M.Y., Min do, S. and Choi, K.Y. (2016) Polygonum aviculare L. and Its Active Compounds, Quercitrin Hydrate, Caffeic Acid, and Rutin, Activate the Wnt/β-catenin Pathway and Induce Cutaneous Wound Healing. Phytotherapy Research, 30, 848-854. https://doi.org/10.1002/ptr.5593
[14]
Hsu, C.Y. (2006) Antioxidant Activity of Extract from Polygonum aviculare L. Biological Research, 39, 281-288. https://doi.org/10.4067/S0716-97602006000200010
[15]
Ma’or, Z., Cohen, D., La’or-Costa, Y. and Portugal-Cohen, M. (2020) Safe Retinol-Like Skin Biological Effect by a New Complex, Enriched with Retinol Precursors. Journal of Cosmetics, Dermatological Sciences and Applications, 10, 59-75. https://doi.org/10.4236/jcdsa.2020.102007
[16]
Cox, W.G. and Singer, V.L. (2004) Fluorescent DNA Hybridization Probe Preparation Using Amine Modification and Reactive Dye Coupling. BioTechniques, 36, 114-122. https://doi.org/10.2144/04361RR02
[17]
Väremo, L., Nielsen, J. and Nookaew, I. (2013) Enriching the Gene Set Analysis of Genome-Wide Data by Incorporating Directionality of Gene Expression and Combining Statistical Hypotheses and Methods. Nucleic Acids Research, 41, 4378-4391. https://doi.org/10.1093/nar/gkt111
[18]
Subramanian, A., Tamayo, P., Mootha, V.K., Mukherjee, S., Ebert, B.L., Gillette, M.A., Paulovich, A., Pomeroy, S.L., Golub, T.R., Lander, E.S. and Mesirov, J.P. (2005) Gene Set Enrichment Analysis: A Knowledge-Based Approach for Interpreting Genome-Wide Expression Profiles. Proceedings of the National Academy of Sciences of the United States of America, 102, 15545-15550. https://doi.org/10.1073/pnas.0506580102
[19]
Ogé, L.K., Broussard, A. and Marshall, M.D. (2019) Acne Vulgaris: Diagnosis and Treatment. American Family Physician, 100, 475-484.
[20]
Heng, A.H.S. and Chew, F.T. (2020) Systematic Review of the Epidemiology of Acne Vulgaris. Scientific Reports, 10, Article No. 5754. https://doi.org/10.1038/s41598-020-62715-3
[21]
Bataille, A., Le Gall-Ianotto, C., Genin, E. and Misery, L. (2019) Sensitive Skin: Lessons from Transcriptomic Studies. Frontiers in Medicine, 6, Article 115. https://doi.org/10.3389/fmed.2019.00115
[22]
Wong, D.J. and Chang, H.Y. (2005) Learning More from Microarrays: Insights from Modules and Networks. Journal of Investigative Dermatology, 125, 175-182. https://doi.org/10.1111/j.0022-202X.2005.23827.x
[23]
Orioli, D. and Dellambra, E. (2018) Epigenetic Regulation of Skin Cells in Natural Aging and Premature Aging Diseases. Cells, 7, Article 268. https://doi.org/10.3390/cells7120268
[24]
Sánchez-Pellicer, P., Navarro-Moratalla, L., Núñez-Delegido, E., Ruzafa-Costas, B., Agüera-Santos, J. and Navarro-López, V. (2022) Acne, Microbiome, and Probiotics: The Gut-Skin Axis. Microorganisms, 10, Article 1303. https://doi.org/10.3390/microorganisms10071303
[25]
Goodarzi, A., Mozafarpoor, S., Bodaghabadi, M. and Mohamadi, M. (2020) The Potential of Probiotics for Treating Acne Vulgaris: A Review of Literature on Acne and Microbiota. Dermatology and Therapy, 33, e13279. https://doi.org/10.1111/dth.13279
[26]
Jo, J.H., Harkins, C.P., Schwardt, N.H., Portillo, J.A.; NISC Comparative Sequencing Program; Zimmerman, M.D., Carter, C.L., Hossen, M.A., Peer, C.J., Polley, E.C., Dartois, V., Figg, W.D., Moutsopoulos, N.M., Segre, J.A. and Kong, H.H. (2021) Alterations of Human Skin Microbiome and Expansion of Antimicrobial Resistance after Systemic Antibiotics. Science Translational Medicine, 13, eabd8077. https://doi.org/10.1126/scitranslmed.abd8077
