Prema 178 onion variety is widely used in production in Burkina Faso. It is greatly appreciated but susceptible to basal rot. This study aimed to evaluate the pathogenic ability of six strains of Fusarium genus identified in Burkina Faso on onion. Seeds, seedlings and bulbs were used for the test. A conidial suspension of each strain was made in tubes and adjusted to 1 × 106 conidia/ml with distilled water for the different tests. Germination test in the laboratory and greenhouse showed that all treatments with the strains of Fusarium oxysporum f. sp. cepae, F. solani, F. falciforme, F. acutatum, F. proliferatum and F. sp. induced failure to emerge and showed a significant difference with the control. The different strains also induced stunting rates of coleoptile growth. Fusarium oxysporum f. sp. cepae, F. acutatum, F. proliferatum, F. falciforme and F. solani were very aggressive, as they recorded above 50% damping-off rates. The test on the bulbs revealed that the strains were classified into two groups. The first consists of F. oxysporum f. sp. cepae, F. solani, F. falciforme, F. acutatum, which caused rots with respective lengths from 2.06; 1.48; 1.84; 1.46 and 2.12 cm, thus very aggressive according to Ghanbarzadeh scale. The second is formed by F. proliferatum which recorded 0.90 cm of rot length, thus moderately aggressive. It would be appropriate to suggest a sustainable management method for these pathogens in order to improve the yield of onion production.
References
[1]
White, K. and Zellner, J. (2008) Onion. Hamilton College Seminar 235 Food for Thought: The Science, Culture and Politics of Food Spring, New York, United States, August 2008, 29.
[2]
Faostat (2020) Food and Agricultural Organization. https://www.fao.org/faostat/fr/#data/QV
[3]
DPSAA (2011) Rapport général du module maraîchage. Ministère de l’Agriculture et de l’Hydraulique, Ouagadougou.
[4]
DGESS (2019) Rapport de l’Enquête maraichère 2018. Ministère de l’agriculture et des aménagements hydro-agricoles, Ouagadougou.
[5]
Özer, N., Köycü, N., Chilosi, G. and Magro, P. (2004) Resistance to Fusarium Basal Rot of Onion in Greenhouse and Field and Associated Expression of Antifungal Compounds. Phytoparasitica, 32, 388-394. https://doi.org/10.1007/BF02979850
[6]
Schwartz, H.F. and Mohan, S.K. (2008) Compendium of Onion and Garlic Diseases and Pests. 2nd Edition, The American Phytopathological Society, St. Paul.
[7]
Somkuwar, R., Veere-Gowda, R., Singh, T.H. and Pathak, C.S. (1996) Screening of Onion for Resistance to Onion Basal Rot. Madras Agricultural Journal, 83, 273-275.
[8]
Köycü, N. and Özer, N. (1997) Determination of Seedborne Fungi in Onion and Their Transmission to Onion Sets. Phytoparasitica, 25, 25-31. https://doi.org/10.1007/BF02981476
[9]
Schwartz, H.F. and Mohan, S.K. (2008) Compendium of Onion and Garlic Diseases and Pests. 2nd Edition, The American Phytopathological Society, St. Paul, 23-25.
[10]
Zlata, K.Š., Jelena, L., Stevan, M., Jelica, G.V., Mirjana, V. and Svjetlana, A. (2008) Fusarium Rot of Onion and Possible Use of Bioproduct. Zbornik Matice Srpske za Prirodne Nauke, 114, 135-148. https://doi.org/10.2298/ZMSPN0814135K
[11]
Pitt, J.I. (2000) Toxigenic Fungi: Which Are Important? Medical Mycology, 38, 17-22. https://doi.org/10.1080/mmy.38.1.17.22
[12]
Kintéga, K.R., Zida, P.E., Tarpaga, V.W., Sankara, P. and Séréme, P. (2020) Identification of Fusarium Species Associated with Onion (Allium cepa L.) Plants in Field in Burkina Faso. Advances in Biosciences and Biotechnology, 11, 94-110. https://doi.org/10.4236/abb.2020.113008
[13]
Sogoba, K.H., Koïta,K., Ouattara, A., Nana, T.A., Soura, B.H. and Campa, C. (2021) Distribution, Diversity and Identification of Hot Spot of Fusarium spp. Associated with Onion (Allium cepa L.) in Burkina Faso. Journal of Agricultural and Crop Research, 9, 241-249.
[14]
Sogoba, K.H., Koïta, K., Ouattara, A., Sana, M., Kassankogno, A.I., Sawadogo, H. and Campa, C. (2022) Pathogenic Characterization of Three Fusarium Species Associated with Onion (Allium cepa L.) in Burkina Faso. International Journal of Phytopathology, 11, 267-276. https://doi.org/10.33687/phytopath.011.03.4327
[15]
Kaboré, K.H. (2014) étude de la pathogénicité et du contrôle de souches d’Alternaria et de Fusarium isolées sur oignon au Burkina Faso. Université Cathologique de Louvain, Faculté d’Ingénierie biologique, agronomique et environnementale, Belgique.
[16]
Kalman, B., Abraham, D., Graph, S., Perl-Treves, R., Harel, Y.M. and Degani, O. (2020) Isolation and Identification of Fusarium spp., the Causal Agents of Onion (Allium cepa L.) Basal Rot in Northeastern Israel. Biology, 9, 69. https://doi.org/10.3390/biology9040069
[17]
Bayraktar, H. and Dolar, F.S. (2010) Molecular Identification and Genetic Diversity of Fusarium Species Associated with Onion Fields in Turkey. Journal of Phytopathology, 159, 28-34. https://doi.org/10.1111/j.1439-0434.2010.01715.x
[18]
Ghanbarzadeh, B., Goltapeh, E.M. and Safaie, N. (2014) Identification of Fusarium Species Causing Basal Rot of Onion in East Azarbaijan Province, Iran and Evaluation of Their Virulence on Onion Bulbs and Seedlings. Archives of Phytopathology and Plant Protection, 47, 1050-1062. https://doi.org/10.1080/03235408.2013.829628
[19]
Tirado-Ramirez, M.A., López-Urquídez, G.A., Amarillas-Bueno, L.A., Retes-Manjarrez, J.E., Vega-Gutiérrez, T.A. and Avendaño, J.E.L. (2021) Identification and Virulence of Fusarium falciforme and Fusarium brachygibbosum as Causal Agents of Basal Rot on Onion in Mexico. Canadian Journal of Plant Pathology, 43, 722-733. https://doi.org/10.1080/07060661.2021.1894241
[20]
Haapalainen, M., Latvala, S., Kuivainen, E., Qiu, Y., Segerstedt, M. and Hannukkala, A.O. (2016) Fusarium oxysporum, F. proliferatum and F. redolens Associated with Basal Rot of Onion in Finland. Plant Pathology, 65, 1310-1320. https://doi.org/10.1111/ppa.12521
[21]
Esfahani, M.N. (2018) Genetic and Virulence Variation in Fusarium oxysporum f. sp. cepae Causing Root and Basal Rot of Common Onion in Iran. Journal of Phtytopatholoy, 166, 572-580.
[22]
Kintéga, K.R., Zida, P.E., Soalla, R., Tarpaga, V.W., Sankara, P. and Sereme, P. (2020) Determination of Fusarium Species Associated with Onion Plants (Allium cepa L.) in Field in Burkina Faso Causing Damping-Off and Bulb Rots. American Journal of Plant Sciences, 11, 64-79. https://doi.org/10.4236/ajps.2020.111006
[23]
Leslie, J.F. and Summerell, B.A. (2006) The Fusarium Laboratory Manual. Blackwell Publishing, Hoboken. https://doi.org/10.1002/9780470278376
[24]
Le, D., Audenaert, K. and Haesaert, G. (2021) Fusarium Basal Rot: Profile of an Increasingly Important Disease in Allium spp. Tropical Plant Pathology, 46, 241-253. https://doi.org/10.1007/s40858-021-00421-9
[25]
O’Donnell, K., Ward, T.J., Vincent, A., Crous, R.P.W., Geiser, D.M. and Kang, S. (2015) DNA Sequence-Based Identification of Fusarium: Current Status and Future Directions. Phytoparasitica, 43, 583-595. https://doi.org/10.1007/s12600-015-0484-z
[26]
Kee, Y.J., Zakaria, L. and Mohd, M.H. (2020) Morphology, Phylogeny and Pathogenicity of Fusarium Species from Sansevieria trifasciata in Malaysia. Plant Pathology, 69, 442-454. https://doi.org/10.1111/ppa.13138
[27]
Dongzhen, F., Xilin, L., Xiaorong, C., Wenwu, Y., Yunlu, H., Yi, C., Jia, C., Zhimin, L., Litao, G., Tuhong, W., Xu, J. and Chunsheng, G. (2020) Fusarium Species and Fusarium oxysporum Species Complex Genotypes Associated with Yam Wilt in South-Central China. Frontiers in Microbiology, 11, Article No. 1964. https://doi.org/10.3389/fmicb.2020.01964
[28]
Ma, L.J., Geiser, D.M., Proctor, R.H., Rooney, A.P., O’Donnell, K., Trail, F., Gardiner, D.M., Manners, J.M. and Kazan, K. (2013) Fusarium Pathogenomics. Annual Review of Microbiology, 67, 399-416. https://doi.org/10.1146/annurev-micro-092412-155650
[29]
Taylor, A., Vagany, V., Jackson, A.C., Harrison, R.J., Rainoni, A. and Clarkson, J.P. (2016) Identification of Pathogenicity-Related Genes in Fusarium oxysporum f. sp. cepae. Molecular Plant Pathology, 17, 1032-1047. https://doi.org/10.1111/mpp.12346
