To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, electrophoresis buffers were used widely to separate any DNA molecule. In this paper, we used four types of previously known electrophoresis buffers to compare which is easy for preparation, simple in structure, low cost and good performance in agarose gel electrophoresis. For this, we used two agarose concentration (1%, 2%) and two types of DNA ladder (100 bp, 1 kb) represent both smaller and larger sizes of molecule for each type of buffers, from the result we found in first level both supper buffer and TAE buffer with good performance and in second level we found bicarbonate buffer also with good performance also. Finally, we found the tang buffer cannot pose any electrophoretic activity on DNA agarose gel electrophoresis.
References
[1]
Sambrook, J. (2001) Molecular Cloning: A Laboratory Manual (in Spanish). Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
[2]
Kryndushkin, D.S., Alexandrov, I.M., Ter-Avanesyan, M.D. and Kushnirov, V.V. (2003) Yeast [PSI+] Prion Aggregates Are Formed by Small Sup35 Polymers Fragmented by Hsp104. Journal of Biological Chemistry, 278, 49636-49643. https://doi.org/10.1074/jbc.M307996200
[3]
Lee, P.Y., Costumbrado, J., Hsu, C.Y. and Kim, Y.H. (2012) Agarose Gel Electrophoresis for the Separation of DNA Fragments. Journal of Visualized Experiments, 62, e3923.
[4]
Stellwagen, E. and Stellwagen, N. (2002) The Free Solution Mobility of DNA in Tris-Acetate-EDTA Buffers of Different Concentrations, with and without Added NaCl. Electrophoresis, 23, 1935-1941.
[5]
Ogden, R.C. and Adam, D.A. (1987) Electrophoresis in Agarose and Acrylamide Gels. Methods in Enzymology, 152, 61-87. https://doi.org/10.1016/0076-6879(87)52011-0
[6]
Zhang, J.-H., Wang, F. and Wang, T.-Y. (2011) A Simple and Effective Super Buffer for DNA Agarose Electrophoresis. Gene, 487, 72-74 https://doi.org/10.1016/j.gene.2011.05.018
[7]
Ens, S., Olson, A.B., Dudley, C., et al. (2012) Inexpensive and Safe DNA Gel Electrophoresis Using Household Materials. Biochemistry and Molecular Biology Education, 40, 198-203. https://doi.org/10.1002/bmb.20596
[8]
Shirazu, Y., Lee, D. and Abd-Elmissih, E. (2009) The MacGyver Project: Genomic DNA Extraction and Gel Electrophoresis Experiments Using Everyday Materials. The Science Creative Quarterly, 4, 1-14.
[9]
Sharp, P.A., Sugden, B. and Sambrook, J. (1973) Detection of Two Restriction Endonuclease Activities in H. parainfluenzae Using Analytical Agarose-Ethidium Bromide Electrophoresis. Biochemistry, 12, 3055-3063. https://doi.org/10.1021/bi00740a018
[10]
Bourzac, K.M., LaVine, L.J. and Rice, M.S. (2003) Analysis of DAPI and SYBR Green I as Alternatives to Ethidium Bromide for Nucleic Acid Staining in Agarose Gel Electrophoresis. Journal of Chemical Education, 80, 1292-1296. https://doi.org/10.1021/ed080p1292
