饲料中添加肽聚糖对中华绒螯蟹幼蟹生长、肝胰腺部分免疫指标及抗病力的影响
Effect of Peptidoglycan on Growth Performance, Some Immune Parameters of Hepatopancreas and Disease Resistance in Juvenile Chinese Mitten Crab (Eriocheir sinensis)

为研究饲料中添加肽聚糖对中华绒螯蟹幼蟹生长及抗氧化酶活力的影响，在基础饲料中分别添加0 (对照组)、62.5 mg/kg、125 mg/kg和250 mg/kg肽聚糖，配制成4组实验饲料，分别投喂初始体质量为(32.7 ± 1.4) g的中华绒螯蟹幼蟹42 d。结果显示，实验蟹的体质量、增重率、特定生长率等生长指标随着饲料中肽聚糖添加量的提高先升后降，在添加量为125 mg/kg时达到最大值，显著高于对照组。相比于对照组，添加125 mg/kg饲料的肽聚糖组实验蟹肝胰腺中蛋白浓度、酸性磷酸酶(ACP)、碱性磷酸酶(ALP)显著提高。攻毒感染试验表明，125 mg/kg肽聚糖组相对免疫保护率最高(38.22%)，显著高于其它组。研究表明，饲料中适量添加肽聚糖可显著提高中华绒螯蟹幼蟹的生长性能、增强抗氧化能力和抗菌能力。
An experiment was conducted to investigate the effects of peptidoglycan content on growth performance and antioxidant activity of Eriocheir sinensis. Peptidoglycan levels of 0, 62.5, 125 and 250 mg/kg were supplemented to basal diet to formulate four diets. Each diet was randomly assigned to triplicate cages of 90 crabs with an initial average weight of (32.7 ± 1.4) g for 42 days. The results showed that the body mass, weight gain, and specific growth rate firstly increased and then decreased with the increase of peptidoglycan, and reached the maximum when the dietary supple-mentation was 125 mg/kg, which were significantly higher than those of the control group. Compared with the control group, protein concentration, ACP and ALP were significantly increased. The artificial infection test shown that the relative immunoprotection rate was highest in group 125 mg/kg, up to 38.22%, which was significantly higher than that in the other groups. All these results indicated that the suitable peptidoglycan supplementation could significantly improve growth performance, enhance the antioxidant capacity and antimicrobial ability.