基于ISSR分子标记的山栀单株遗传多样性研究
Genetic Diversity Study of Gardenia jasminoides Ellis Single Plants Based on ISSR Molecular Marker Analysis

山栀是药食同源的经济林，主要含有环烯醚萜类、色素、胆碱等活性成分，是重要的药物资源，也是化工、食品工业的重要原料，其中天然栀子黄色素是我国传统染料，作为天然食品添加剂，具有较高的经济价值。实验挑选湖南长沙宁乡的优质栀子提取DNA为材料，筛选出U840为引物，采用ISSR分子标记方法对其遗传多样性进行分析，通过NTSYS和POPGEN软件对其进行聚类分析和遗传多样性分析，得到此居群的种质资源情况，可为下一步进行湖南山栀其他居群的种质资源研究打下基础，并为新品种选育提供科学依据和技术支撑。
Gardenia jasminoides Ellis is an economic forest of affinal drug and diet, mainly containing iridoids, pigments, choline and other active ingredients. It is an important drug resource and an important raw material for chemical industry and food industry. Among them, natural gardenia yellow pig-ment is a traditional dye in China, as a natural food additive, has high economic value. In this ex-periment, high-quality gardenia DNAs from Ningxiang, Changsha, Hunan province were selected as materials, and U840 was selected as primer. Their genetic diversity was analyzed by ISSR molecular marker method. Cluster analysis and genetic diversity analysis were analyzed by NTSYS and POPGEN software to obtain the germplasm resources of this population. It can lay the groundwork for the next research on germplasm resources in other gardenia populations in Hunan, and provide scientific basis and technical support for the breeding of new varieties.