米槁成熟种子长期保存研究
Study on Long-Term Cryopreservation Preservation with Mature Seeds of Cinnamomum migao

研究种质的保存问题，对濒危植物的保护和种质资源的长期利用尤为重要。方法：以来自贵州省三个不同种群的米槁成熟种子为研究对象，对干燥脱水后的种子活力、萌发以及低温保存后的种子活力、萌发进行测试。结果：刚采集回来的米槁种子直接播种萌发率极低，但种子活力值高，将新鲜种子胚轴剥离进行离体萌发，胚胎体外发芽率最高有95%；进行干燥脱水后的种子均不能萌发，但剥离出胚胎后可以萌发；胚胎体外发芽率随含水量的减少而降低，当种子脱水至最低含水量时，胚胎体外发芽率最低为坝布种群12.5%，最高为巧乱种群65%；低温保存后解冻恢复48 h的米槁种子进行TTC染色和胚胎体外发芽实验，结果表明胚胎活力值为0%，胚胎体外发芽率为0%。结论：米槁成熟种子的胚胎具有一定的耐脱水性，但种子不耐脱水；经干燥脱水后的米槁成熟种子是不能进行低温保存的，这为米槁种子的长期储存提供了科学依据。
Research on the conservation of germplasm is particularly important for the protection of endangered plants and the long-term utilization of germplasm. Method: The mature seeds of Cinnamomum migao from three different populations in Guizhou Province were studied. Seed viability and germination after drying and dehydration, and the seed viability and germination after cryopreservation were tested. The germination of the freshly collected rice seeds is extremely low, but the seed vitality value is high. The embryo germination rate was up to 95% in vitro by peeling off the cotyl of fresh seeds. Seeds cannot germinate after dehydration, but the embryos can germinate. The germination rate of embryos in vitro decreases with the decrease of water content. When the seeds were dehydrated to the lowest water content, the embryo germination rate in vitro was as low as 12.5% of the Babu population and 65% at the highest in the Qiaoluan population. After thawing, the seed vitality was 0% by determining with TTC method and in vitro germination of plumular axis was 0% of C. migao. Conclusion: The embryos of mature seeds have a certain degree of resistance to dehydration, but the seeds are not resistant to dehydration. After drying and dehydration, mature seeds cannot be stored at cryopreservation, which provides a scientific basis for the germplasm storage of C. migao seeds.