In this study, an effective environment for Chlorella vulgaris growth is sought after. As a substitute source of food and feed, increasing the
cell density of Chlorella culture is one of the keys to ensuring
sustainability. It can be showed from different studies that optimum
light intensity and pH could increase cell density. In this study, the effects
of light and pH on the growth rate of C. vulgaris were observed in
photobioreactor. A specific wavelength (682 nm) was determined by UV-Vis
Spectrophotometry to carry out the further analysis. The light intensities were
set at 7409, 9261 and 11,113 lux; pH values were set at 7, 8 and 9
respectively. The experimental results depicted the light intensity of 9261 lux as the best due to the higher number
of cells (48.56 × 106 cells/mL) obtained using this
intensity. In terms of pH, without pH control, cell numbers were found to be
highest under the light intensity of 9261 lux. When pH was controlled, it was
found that under the optimum light intensity, pH control between 7.0 and 7.5 was
the optimum range for the growth of C. vulgaris.Moreover, this method of study may possibly be a
promising source of low cost culture for Chlorella vulgaris.
References
[1]
Khan, M.I., Shin, J.H. and Kim, J.D. (2018) The Promising Future of Microalgae: Current Status, Challenges and Optimization of a Sustainable and Renewable Industry for Biofuels, Feed and Other Products. Microbial Cell Factories, 17, 36.
https://doi.org/10.1186/s12934-018-0879-x
[2]
Sharif, A.M., Shekhar, R.B. and Manzoor, R. (2018) Biodiversity in Bangladesh. Global Biodiversity, 1, 93-107. https://doi.org/10.1201/9780429487743-3
[3]
Gilbert, R. and Muhammad, A.A. (2017) Microalgae: A Potential Plant for Energy Production. Geology, Ecology and Landscapes, 1, 104-120.
https://doi.org/10.1080/24749508.2017.1332853
[4]
Apriliyanti, S., Soeprobowati, T.R. and Yulianto, B. (2016) The Relationship of Chlorella sp. Abundance with the Quality of the Aquatic Environment on a Semi-Mass Scale at BBBPBAP Jepara. Jurnal Ilmu Lingkungan, 14, 77-81.
https://doi.org/10.14710/jil.14.2.77-81
[5]
Biolita, O.N. and Harmadi (2017) Design of Chlorella vulgaris Microalgae Photobioreactor to Optimize Oxygen (O2) Concentration. Jurnal Fisika Unand, 6, 296-305.
https://doi.org/10.25077/jfu.6.3.296-305.2017
[6]
Ördög, V., Stirk, W.A., Lenobel, R., Bancí?ová, M., Strnad, M., Van Staden, J., Szigeti, J. and Németh, J. (2004) Screening Microalgae for Some Potentially Useful Agricultural and Pharmaceutical Secondary Metabolites. Journal of Applied Phycology, 16, 309-314. https://doi.org/10.1023/B:JAPH.0000047789.34883.aa
[7]
Prihantini, N.B., Rachmayanti, W. and Wardhana, W. (2019) Effect of Photoperiodicity Variations on Chlorella Growth on Bold Basal Medium. Jurnal Biota, 12, 32-39. https://doi.org/10.24002/biota.v12i1.2534
[8]
Widyastuti, R.C. and Dewi, A.C. (2015) Sintesis Biodiesel Dari Minyak Mikroalga Chlorella vulgaris Dengan Reaksi Transesterifukasi Mengguanakan Katalis KOH. Jurnal Bahan Alam Terbarukan, 4, 1-12.
[9]
Anupama and Ravindra, P. (2000) Value-Added Food: Single Cell Protein. Biotechnology Advances, 18, 459-479. https://doi.org/10.1016/S0734-9750(00)00045-8
[10]
Padovan, A. (1992) Isolation and Culture of Five Species of Freshwater Algae from the Alligator Rivers Region, Northern Territory. Technical Memorandum No. 37. Australian Government Publishing Service, Canberra.
[11]
Xu, Y., Ibrahim, I.M. and Harvey, P.J. (2016) The Influence of Photoperiod and Light Intensity on the Growth and Photosynthesis of Dunaliella salina (Chlorophyta) CCAP 19/30. Plant Physiology and Biochemistry, 106, 305-315.
https://doi.org/10.1016/j.plaphy.2016.05.021
[12]
Difusa, A., Talukdar, J., Kalita, M.C., Mohanty, K. and Goud, V.V. (2015) Effect of Light Intensity and pH Condition on the Growth, Biomass and Lipid Content of Microalgae Scenedesmus Species. Biofuels, 6, 37-44.
https://doi.org/10.1080/17597269.2015.1045274
[13]
Lee, E., Jalalizadeh, M. and Zhang, Q. (2015) Growth Kinetic Models for Microalgae Cultivation: A Review. Algal Research, 12, 497-512.
https://doi.org/10.1016/j.algal.2015.10.004
[14]
Khalil, Z.I., Asker, M.M., EI-Sayed, S. and Kobbia, I.A. (2010) Effect of pH on Growth and Biochemical Responses of Dunaliella bardawil and Chlorella ellipsoidea. World Journal of Microbiology and Biotechnology, 26, 1225-1231.
https://doi.org/10.1007/s11274-009-0292-z
[15]
Bischoff, H.W. and Bold, H.C. (1963) Phycological Studies IV. Some Soil Algae from Enchanted Rock and Related Algal Species. Publication No. 6318, University of Texas, Austin, 95.
[16]
Environmental Protection Agency (EPA) United States (1994) Short-Term Methods for Measuring the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms. 3rd Edition (EPA 600/4-91/002).
[17]
Hill, C., Willoughby, N. and Bridle, H. (2022) Efficient High-Concentration Dewatering of Chlorella vulgaris Utilizing Spiral Inertial Microfluidics. Bioresource Technology Reports, 18, Article ID: 101014. https://doi.org/10.1016/j.biteb.2022.101014
[18]
Kondzior, P. and Butarewicz, A. (2021) Influence of Walls in a Container on the Growth of the Chlorella vulgaris Algae. Journal of Ecological Engineering, 22, 98-108.
https://doi.org/10.12911/22998993/142185
[19]
Rodrigues, L.H.R., Arenzon, A., Raya-Rodriguez, M.T. and Fontoura, N.F. (2011) Algal Density Assessed by Spectrophotometry: A Calibration Curve for the Unicellular Algae Pseudokirchneriella subcapitata. Journal of Environmental Chemistry and Ecotoxicology, 3, 225-228. https://doi.org/10.5897/JECE2011.025
[20]
Dean, A., Sigee, D., Estrada, B. and Pittman, J. (2010) Using FTIR Spectroscopy for Rapid Determination of Lipid Accumulation in Response to Nitrogen Limitation in Freshwater Microalgae. Bioresource Technology, 101, 4499-4507.
https://doi.org/10.1016/j.biortech.2010.01.065
[21]
Sharma, R., Singh, G.P. and Sharma, V.K. (2012) Effects of Culture Conditions on Growth and Biochemical Profile of Chlorella vulgaris. Journal of Plant Pathology & Microbiology, 3, Article ID: 1000131. https://doi.org/10.4172/2157-7471.1000131
[22]
Daliry, S., Hallajisani, A., Mohammadi, R.J., Nouri, H. and Golzary, A. (2017) Investigation of Optimal Condition for Chlorella vulgaris Microalgae Growth. Global Journal of Environmental Science and Management, 3, 217-230.
[23]
Singh, S.P. and Singh, P. (2015) Effect of Temperature and Light on the Growth of Algae Species: A Review. Renewable and Sustainable Energy Reviews, 50, 431-444.
https://doi.org/10.1016/j.rser.2015.05.024
[24]
Li, Y., Zhou, W., Hu, B., Min, M., Chen, P. and Ruan, R.R. (2012) Effect of Light Intensity on Algal Biomass Accumulation and Biodiesel Production for Mixotrophic Strains Chlorella kessleri and Chlorella protothecoide Cultivated in Highly Concentrated Municipal Wastewater. Biotechnology and Bioengineering, 109, 2222-2229.
https://doi.org/10.1002/bit.24491
[25]
Khoeyi, Z.A., Seyfabadi, J. and Ramezanpour, Z. (2010) Effect of Light Intensity and Photoperiod on Biomass and Fatty Acid Composition of the Microalgae, Chlorella vulgaris. Aquaculture International, 20, 41-49.
https://doi.org/10.1007/s10499-011-9440-1
[26]
Seyfabadi, J., Ramezanpour, Z. and Khoeyi, Z.A. (2011) Protein, Fatty Acid, and Pigment Content of Chlorella vulgaris under Different Light Regimes. Journal of Applied Phycology, 23, 721-726. https://doi.org/10.1007/s10811-010-9569-8
[27]
Muchammad, A., Kardena, E. and Rianti, A. (2013) Pengaruh Intensitas Cahaya Terhadap Penyerapan Gas Karbondioksida Oleh Mikroalga Tropis Ankistrodesmus sp. Dalam Fotobioreaktor.. Jurnal Teknik Lingkungan, 19, 103-111.
https://doi.org/10.5614/jtl.2013.19.2.1
[28]
Febrieni, V.N., Sedjati, S. and Yudiati, E. (2020) Optimization of Light Intensity on Growth Rate and Total Lipid Content of Chlorella vulgaris. IOP Conference Series: Earth and Environmental Science, 584, Article ID: 012040.
https://doi.org/10.1088/1755-1315/584/1/012040
[29]
Wu, H. (2016) Effect of Different Light Qualities on Growth, Pigment Content, Chlorophyll Fluorescence, and Antioxidant Enzyme Activity in the Red Alga Pyropia haitanensis (Bangiales, Rhodophyta). BioMed Research International, 2016, Article ID: 7383918. https://doi.org/10.1155/2016/7383918
[30]
Gong, Q., et al. (2014) Effect of Light and pH on Cell Density of Chlorella vulgaris. Energy Procedia, 61, 2012-2015. https://doi.org/10.1016/j.egypro.2014.12.064
[31]
Widyaningrum, N.F., Susilo, B. and Hermanto, M.B. (2013) Studi Eksperimental Fotobioreaktor Photovoltaic Untuk Produksi Mikroalga (Nannochloropsis oculata). Jurnal Bioproses Komoditas Tropis, 1, 30-38.
