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tRF-His-GTG-037在血管平滑肌细胞表型转换中的表达及生物信息学分析
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Abstract:
目的：探讨tRF-His-GTG-037在血管平滑肌细胞(vascular smooth muscle cell, VSMC)表型转换中的表达水平并利用生物信息学分析其潜在的作用。方法：利用荧光定量PCR检测tRF-His-GTG-037在VSMC表型转化过程中的表达水平。基于miRanda和TargetScan预测tRF-His-GTG-037的靶基因。对预测的靶基因进行GO (Gene Ontology)功能分析和KEGG(Kyoto Encyclopedia of Genes and Genomes)通路分析。结果：与正常VSMC (1.18 ± 0.25)相比，tRF-His-GTG-037在血小板衍生因子BB (platelet-derived growth factor BB, PDGF-BB)诱导VSMC中的表达水平(2.50 ± 0.40)明显升高(t = 2.78, P = 0.008)。预测tRF-His-GTG-037共有93个靶基因，其中49个基因与心血管疾病发病有关，Rac1、组织因子途径抑制物(tissue factor pathway inhibitor, TFPI)、细胞色素P450家族成员1B1 (recombinant cytochrome P450 1B1, CYP1B1)、TWIST1与VSMC的增殖、迁移有关。GO分析结果发现生物过程(biological process, BP)富集项目429个，细胞成分(cell composition, CC)富集项目50个，分子功能(molecular function, MF)富集项目29个。KEGG通路分析结果发现的cAMP信号通路、AMPK信号通路与VSMC的增殖、迁移有关。结论：tRF-His-GTG-037在VSMC表型转换表达水平增高，可能作用于Rac1、TFPI、CYP1B1、TWIST1等靶基因通过cAMP信号通路、AMPK信号通路在VSMC表型转换过程中发挥作用。
Objective: To investigate the expression level of tRF-His-GTG-037 in the phenotypic transformation of vascular smooth muscle cells (VSMCs) and analyze t its potential role with bioinformatics. Methods: The expression level of tRF-His-GTG-037 in VSMC phenotype transformation was detected by fluorescence quantitative PCR. The target gene of tRF-His-GTG-037 was predicted based on miRanda and TargetScan. The predicted target genes were analyzed for GO (Gene Ontology) function and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway. Results: Compared with normal VSMC (1.18 ± 0.25), the expression level of tRF-His-GTG-037 in PDGF-BB (platelet-derived growth factor BB) induced VSMC (2.50 ± 0.40) was significantly higher (t = 2.78, P = 0.008). It was predicted that there were 93 target genes in tRF-His-GTG-037, 49 of which were related to the pathogenesis of cardiovascular disease. Rac1, tissue factor pathway inhibitor (TFPI), recombinant cytochrome P450 1B1 (CYP1B1) and TWIST1 are related to the proliferation and migration of VSMC. The results of GO analysis found 429 biological process (BP) enrichment projects, 50 cell composition (CC) enrichment projects, and 29 molecular function (MF) enrichment projects. The results of KEGG pathway analysis showed that the cAMP signal pathway and AMPK signal pathway were related to the proliferation and migration of VSMC. Conclusions: The expression level of tRF-His-GTG-037 in VSMC phenotypic transformation increased. tRF-His-GTG-037 may play a role in VSMC phenotypic transformation by targeting genes such as Rac1, TFPI, CYP1B1 and TWIST1 through cAMP signal pathway and AMPK signal pathway.
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