White tea encompasses a number of teas unique to eastern Fujian in China.
Although white tea extracts have been reported to result in cancer cell
apoptosis, to date, few studies have evaluated the mechanism of such apoptotic
induction. A transcription factor that plays a critical role in cell apoptosis,
NF-κB p65, is also likely critical by
which white tea extracts induce cancer cell apoptosis. In this study, white tea
aqueous extract (WTAE) was added to BEL-7402 and Hela cell media and NF-κB p65 activation was evaluated using
western blotting and immunofluorescence. Results revealed that the
phosphorylation of IKBα and p65 decreased
in both cell lines after WTAE treatment. And the nuclear translocation of NF-κB p65 in both cell lines was also
reduced with the WTAE treatment. NF-κB
p65 inhibition was noted to accelerate apoptosis. Our findings suggest that NF-κB p65 was an important modulator in
WTAE-induced apoptotic signal transduction and it acted as a negative regulator
of apoptotic induction in BEL-7402 and Hela cancer cell lines.
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