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清营汤可减轻SAP大鼠肠屏障氧化应激损伤
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Abstract:
重症急性胰腺炎(Severe Acute Pancreatitis, SAP)常伴随肠屏障功能损伤，从而恶化预后，但其机制不明确，治疗方式不统一。本研究的目的是探究通过服用古方清营汤能否减轻这一损伤，并观察其对肠粘膜机械屏障的作用。采用逆行胆管注射5%牛磺胆酸钠建立SAP模型。将大鼠分为3组：对照组(SO)、SAP组(SAP)、清营汤干预组(QYT)。检测血清淀粉酶、脂肪酶、TNF-α等指标评估炎症严重程度。HE染色观察肠道组织学变化；采用超氧化物歧化酶(SOD)和谷胱甘肽(GSH)检测肠上皮细胞氧化应激；检测肠屏障相关蛋白的表达。结果显示，QYT组血清指标、组织损伤严重程度、氧化应激水平均显著低于SAP组同时肠屏障相关蛋白表达回升。这些结果表明清营汤可减轻SAP大鼠肠屏障氧化应激损伤，为该病的治疗提供新思路。
Severe Acute Pancreatitis (SAP) is often accompanied by intestinal barrier dysfunction, which worsens the prognosis, but its mechanism is not clear, and the treatment is not uniform. The aim of this study is to investigate whether Qingyingtang, an ancient prescription, can alleviate this injury and to observe its effect on the intestinal mucosal mechanical barrier. The SAP model was estab-lished by retrograde injection of 5% sodium taurocholate into the bile duct. The rats were divided into three groups: control group (SO), SAP group (SAP) and Qingying tang intervention group (QYT). Serum amylase, lipase, TNF-α and other indicators were detected to evaluate the severity of in-flammation. HE staining was used to observe intestinal histological changes. Superoxide dismutase (SOD) and glutathione (GSH) were used to detect oxidative stress of intestinal epithelial cells. The expression of intestinal barrier related proteins was detected. The results showed that the serum indexes, tissue injury severity and oxidative stress level in QYT group were significantly lower than those in SAP group, and the expression of intestinal barrier related proteins increased. These re-sults indicate that Qingyingtang can reduce oxidative stress injury of intestinal barrier in SAP rats, and provide a new idea for the treatment of SAP.
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