Background:HLA alleles may have association with overall JIA including specific
sub-types. Determation of HLA DRB1, DPB1, DQA1, DQB1 and B27, may be helpful to
diagnose JIA cases where diagnostic dilemma is present.The aim of the study was to find out the association of HLA alleles with
JIA and its subtypes. Methods: This cross sectional study was conducted
in the department of Pediatrics, Bangabandhu Sheikh Mujib Medical University
(BSMMU), Dhaka, from April 2020 to March 2021. A total of 50 cases and 25controlsattending pediatric rheumatology clinic and
pediatric OPD were enrolled in the study.
Data were collected by a pre-designed questionnaire containing
socio-demographic, clinical and laboratory parameters. HLA DR-B1, DQ-A1, DQ-B1
and B27 typing were done for the cases and controls from blood samples by polymerase chain reaction
sequence specific primer (PCR-SSP)
method. Data were analyzed by SPSS
version 26. Frequency, percentage, chi square test and Fisher exact tests were
done for statistical analysis. Results: HLA DR-B1_10 had significant
positive association whereas HLA DR-B1_03 and B1_16
had negative association with overall JIA cases. Poly-articular JIA,
oligo-articular JIA and ERA had association with HLA DRB1_01, B1_08 and B1_12
respectively. HLA DQA1-01 and A1_02 had positive association. However, DQ-A1_06
was negatively associated with JIA cases. Oligo JIA was positively associated
with HLA DQ-A1_01, whereas poly JIA and ERA had association with A1_02. Though,
HLA DQ_B1 had no association with overall JIA cases, analysis showed
association of poly JIA with DQ-B1_04. Frequency of HLA DQ-B1_02 and 03 were
higher among ERA cases, though not significant, but they had strong association
with HLA B27. Conclusion: HLA associations were present with overall JIA
and each sub-groups had different patterns of HLA associations including some
protective roles.
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