Telomeres cap ends of eukaryotic
chromosomes prevent them from degradation and ensure genomic
stability. Cdc13 is an essential telomere recruitment and maintenance protein. A temperature-sensitive point mutation in cdc13
gene leads to telomere impairment, giving rise to cdc13-1 mutants that
suffer lethality at enhanced temperatures. Deleting Exo1 gene from these mutants,
however, leads to the emergence of temperature-tolerant mutants called survivors.
Yeasts are known to exist as either diploids or haploids. These yeastgenotypes
generate survivors. The frequency of survivorship inthe haploid genotype is one cell in 104 cells/generation
at 36°C, however,
the frequency at which they emerge in their diploid counterparts at the same temperature is not known. In this study, we investigated the frequency
of Survivorship in heterozygous diploids of cdc13-1exo1Δmutants of S. cerevisiae at 36°C. Diploids were constructed by mating haploid strains of opposite mating type
cdc13-1 exo1:LEU strains with strains of cdc13-1 exo1:HIS. The crosses were 1296×3181, 2561×3182, 1296×3182 and
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