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Botanical Research 2022
小麦DUF642基因全基因组鉴定及转录组分析
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Abstract:
【目的】分析小麦细胞壁相关蛋白DUF642 (Domain of Unknown Function 642)基因家族的特点。【方法】在小麦全基因组内对DUF642家族成员进行鉴定，并且对其进化关系、基因结构、蛋白特性、保守基序、顺式作用元件和转录组信息进行系统分析。【结果】TaDUF642家族中共有34个家族成员，多物种系统进化树分析、基因结构和保守基序显示，TaDUF642家族成员在进化上具有较高的保守性；蛋白特征显示，大多数TaDUF642蛋白为酸性蛋白质和亲水性蛋白质；亚细胞定位预测显示，TaDUF642大多定位在叶绿体；转录组数据分析显示，TaDUF642基因广泛参与小麦的生长发育和非生物胁迫，并且与禾谷镰刀菌、条锈菌和白粉菌的侵染紧密相关。【结论】本研究精准鉴定了小麦TaDUF642基因家族成员及其表达模式，为小麦在抗逆方面的遗传改良提供了理论依据。
[Objective] Further analyze the properties of DUF642 (Domain of Unknown Function 642) gene family related cell wall in wheat. [Method] The DUF642 family members were identified throughout the whole wheat genome, and their evolutionary relationships, gene structure, protein characteristics, conserved motifs, cis-acting elements and transcriptome information were systematically analyzed. [Result] There were 34 family members in TaDUF642 family. Phylogenetic tree analysis, gene structure and conserved motifs showed that TaDUF642 family members were highly conserved in evolution. Protein characteristics showed most of the TaDUF642 proteins were acidic and hydrophilic. Subcellular localization prediction indicated that TaDUF642 was mostly localized in chloroplast. The analysis of transcriptome data suggested that TaDUF642 genes is widely involved in the growth and development and abiotic stress of wheat, and is closely related to the infection of Fusarium graminis, stripe rust and powdery mildew. [Conclusion] Members of the TaDUF642 genes family and their expression patterns were accurately identified in wheat, which provided a theoretical basis for the genetic improvement of wheat resistance to stress.
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