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Botanical Research 2022
森林草莓柠檬酸合酶基因的表达分析
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Abstract:
我们解析了森林草莓的1个线粒体柠檬酸合酶基因(Fvmcs)和1个乙醛酸循环体柠檬酸合酶基因(Fvgcs)。它们具有柠檬酸合酶基因特有的结构和功能域，并且与其它植物的柠檬酸合酶有很高的序列相似性。在各种组织以及不同发育阶段的果实中，2个柠檬酸合酶基因都是组成型的表达。本研究为提高草莓果实品质和植物抗逆性提供科学依据。
We resolved one mitochondrial citrate synthase gene and one glyoxysomal citrate synthase gene in the woodland strawberry. They have the structural and functional domains unique to the citrate synthase gene, and have high sequence similarity to the citrate synthase from other plants. Two citrate synthase genes are constitutively expressed in various tissues as well as in fruits at different developmental stages. This study provides a scientific basis for improving strawberry fruit quality and plant stress resistance.
| [1] | 周先艳, 朱春华, 李进学，等. 果实有机酸代谢研究进展[J]. 中国南方果树, 2015, 44(1): 120-125+132. |
| [2] | 倪溢楠. 草莓果实品质评价及香气资源挖掘[D]: [硕士学位论文]. 扬州: 扬州大学, 2021. |
| [3] | 刘士华, 吕国胜. 草莓果实成熟过程中糖、酸含量的动态变化[J]. 安徽林业科技, 2011, 37(5): 24-27. |
| [4] | 杨雷, 杨莉, 李莉, 等. 草莓果实重要性状动态规律研究[J]. 华北农学报, 2010, 25(z1): 96-99. |
| [5] | 邵晓庆, 贠建民, 艾对元, 等. 野生草莓果实品质性状的研究进展[J]. 中国酿造, 2014, 33(4): 19-22. |
| [6] | Shulaev, V., Sargent, D.J., Crowhurst, R.N., et al. (2011) The Genome of Woodland Strawberry (Fragaria vesca). Nature Genetics, 43, 109-116. https://doi.org/10.1038/ng.740 |
| [7] | 缪军, 马秀明, 孙杨，等. 草莓种子实生苗病毒分子检测[J]. 现代农业科技, 2022(2): 41-44+50. |
| [8] | 王滕旭, 李正国, 杨迎伍, 等. 甜橙柠檬酸合酶基因的克隆及其表达分析[J]. 中国农学通报, 2010, 26(10): 65-69. |
| [9] | Iannetta, P.P., Escobar, N.M., Ross, H.A., et al. (2004) Identification, Cloning and Expression Analysis of Strawberry (Fragaria × ananassa) Mitochondrial Citrate Synthase and Mitochondrial Malate Dehydrogenase. Physiologia Plantarum, 121, 15-26. https://doi.org/10.1111/j.0031-9317.2004.00302.x |
| [10] | Pracharoenwattana, I., Cornah, J.E. and Smith, S.M. (2005) Arabidopsis Peroxisomal Citrate Synthase Is Required for Fatty Acid Respiration and Seed Germination. The Plant Cell, 17, 2037-2048. https://doi.org/10.1105/tpc.105.031856 |
| [11] | 迟光红, 周雪丽, 李美英, 等. 香蕉柠檬酸合酶基因MaGCS的克隆及生物信息学分析[J]. 热带农业科学, 2009, 29(8): 12-18. |
| [12] | 甘智才, 陈东颖, 张丽，等. 转柠檬酸合成酶基因苜蓿耐铝性研究[J]. 中国农业科学, 2010, 43(16): 3461-3466. |
| [13] | Larsen, P.B., Degenhardt, J., Tai, C.Y., et al. (1998) Aluminum-Resistant Arabidopsis Mutants that Exhibit Altered Patterns of Aluminum Accumulation and Organic Acid Release from Roots. Plant Physiology, 117, 9-18.
https://doi.org/10.1104/pp.117.1.9 |
| [14] | Koyama, H., Kawamura, A., Kihara, T., et al. (2000) Overexpression of Mitochondrial Citrate Synthase in Arabidopsis thaliana Improved Growth on a Phosphorus-Limited Soil. Plant & Cell Physiology, 41, 1030-1037.
https://doi.org/10.1093/pcp/pcd029 |
