Dallol Maouri is one of the fossil valleys on the left bank of the Niger River. Its watershed drains water from valleys in the Tahoua and Dosso regions of Niger. The watercourse is not continuous and has areas of surface water accumulation (ponds) and areas of spreading and infiltration. The surface water potential in this area is limited, with most of the ponds having a temporary to the semi-permanent regime. Groundwater resources are, therefore, the main sources of withdrawal for human food, agriculture, and livestock. Climate change through the variation in rainfall, the often-anarchic multiplication of catchment works (wells and boreholes) and agricultural activities can have impacts on the qualitative and quantitative evolution of surface and groundwater. The objective of this study is to characterize the surface and underground water of the upper dallol Maouri through the physicochemical parameters of the water and the use of the isotopes of the water molecule (18O, 2H and 3H). A total of 53 water samples were analyzed at the laboratory of the Regional Directorate of Hydraulics and Sanitation of Tillabéri (Niger) for chemical parameters and 73 samples were analyzed at the Radio Analysis and Environment Laboratory (LRAE) of the National School of Engineers of Sfax (ENIS) of Tunis for isotopy. The water of the ponds is characterized by three types of facies: calcium bicarbonate, calcium and magnesium bicarbonate, and calcium nitrate chloride. The groundwater is 75% of the chloride-nitrate-calcium facies and the CT3/CT2 groundwater is calcium and magnesium bicarbonate. The results of the stable isotopes made it possible to highlight the presence of four distinct water groups: ponds characterized by highly evaporated water, the sheets of Continental Terminal 1 and 2 (CT1 and CT2) with an isotopic cachet of old water, the water table consisting of a two-layer system in the southwestern part of the area. The water is not very mineralized (40 μS to 600 μS) and is slightly acidic (pH = 6.4). The analysis of the radioactive isotope (3H) content of the water from the water table has made it possible to identify the areas of recharge of the water table. These results also highlight: the presence of an upward drainage zone, and the contribution of lowland ponds to the recharge of alluvial aquifers ranging from 24% to 84%. No structure in this zone reaches the Continental Intercalaire and the Continental Hamadien, the depth of which is estimated at an average of 600 m. The relationship between the 18O and
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