Small RNAs in Penicillium
digitatum were identified and analyzed via transcriptome sequencing on the
BGISEQ-500 platform. A total of 15 predicted miRNAs and 10718 novel siRNAs were
found. Their length distribution, sequence, predicted construction, base bias,
expression levels and potential targets were determined as well. Through
pathway and KEGG enrichment analysis, the miRNA target genes were mostly involved in
carbohydrate metabolism, transport and catabolism, translation and amino acid
metabolism. The target genes involved in aflatoxin biosynthesis and proteasome
had a higher rich factor value. The results will provide a theoretical foundation
for understanding the developmental and pathogenic mechanisms of P. digitatum at the transcriptional
level.
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