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Efficacy of Afoxolaner Alone or in Combination with Milbemycin Oxime against Rhipicephalus sanguineus Lato Sensu in Naturally Infested Dogs in Colombia

DOI: 10.4236/ojvm.2022.124004, PP. 27-36

Keywords: Afoxolaner, Milbemycin Oxime, Rhipicephalus sanguineus, Dogs, Shelter

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The brown dog tick (Rhipicephalus sanguineus lato sensu) is the tick that most affects dogs worldwide and is therefore the main blood pathogen vector in dogs. The efficacy of afoxolaner 2.7 to 7.1 mg/kg NexGard® (group A) and afoxolaner plus milbemycin oxime 2.5 to 5.4 mg/kg and 0.5 to 1.1 mg/kg respectively NexGard Spectra® (Group B) against R. sanguineus, was evaluated in naturally infected sheltered dogs under high challenging conditions in four different areas of Colombia (Antioquia, Córdoba, Santander, and Meta). Tick counts (alive, dead, attached, and unattached) were performed on treated dogs, the average was calculated for the different areas to evaluate the efficacy of each treatment at six different times post-treatment (24 h, 48 h, 7 d, 14 d, 21 d, 30 d). None of the dogs showed adverse events related to the treatments. The average tick number pre-treatment was 68 in group A and 78.3 for group B indicating a strong natural infection of the dogs and their environment. Efficacy after 24 h against R. sanguineus was always above 90% with ≥97.4% for NexGard® and ≥93.7% for NexGard Spectra®. Consistent results were observed along all the observation periods with final efficacies (day 30) of ≥99.8% and 98.3% for NexGard® and NexGard Spectra®, respectively. In conclusion, both NexGard® and Nexgard Spectra® provided a curative effect and sustained efficacy against Rhipicephalus sanguineus for at least 30 days in highly contaminated shelter environments.


[1]  Allen, K., Little, S., Petersen, M., Gruntmeir, J., Barrett, A., Herrin, B., Starkey, L., Sun, F. and Guerino, F. (2020) Evaluation of Oral Fluralaner (Bravecto®) for Efficacy against Nymphs of Amblyomma americanum and Rhipicephalus sanguineus (sensu lato). Parasites and Vectors, 13, Article No. 315.
[2]  Boulanger, N., Boyer, P., Talagrand-Reboul, E. and Hansmann, Y. (2019) Ticks and Tick-Borne Diseases. Médecine et Maladies Infectieuses, 49, 87-97.
[3]  Chomel, B. (2011) Tick-Borne Infections in Dogs—An Emerging Infectious Threat. Veterinary Parasitology, 179, 294-301.
[4]  Gray, J., Dantas-Torres, F., Estrada-Peña, A. and Levin, M. (2013) Systematics and Ecology of the Brown Dog Tick, Rhipicephalus sanguineus. Ticks and Tick-Borne Diseases, 4, 171-180.
[5]  Maggi, R.G. and Krämer, F. (2019) A Review on the Occurrence of Companion Vector-Borne Diseases in Pet Animals in Latin America. Parasites and Vectors, 12, Article No. 145.
[6]  Otranto, D., Dantas-Torres, F. and Breitschwerdt, E.B. (2009) Managing Canine Vector-Borne Diseases of Zoonotic Concern: Part One. Trends in Parasitology, 25, 157-163.
[7]  Day, M.J. (2011) One Health: The Importance of Companion Animal Vector-Borne Diseases. Parasites and Vectors, 4, Article No. 49.
[8]  Dantas-Torres, F. (2010) Biology and Ecology of the Brown Dog Tick, Rhipicephalus sanguineus. Parasites and Vectors, 3, Article No. 26.
[9]  Tielemans, E., Pfefferkorn, A. and Viljoen, A. (2021) Efficacy of a Novel Topical Combination of Esafoxolaner, Eprinomectin and Praziquantel against Rhipicephalus sanguineus in Cats. Parasite, 28, Article No. 24.
[10]  Dumont, P., Blair, J., Fourie, J.J., Chester, T.S. and Larsen, D.L. (2014) Evaluation of the Efficacy of Afoxolaner against Two European Dog Tick Species: Dermacentor reticulatus and Ixodes ricinus. Veterinary Parasitology, 201, 216-219.
[11]  Kondo, Y., Kinoshita, G., Drag, M., Chester, T.S. and Larsen, D. (2014) Evaluation of the Efficacy of Afoxolaner against Haemaphysalis longicornis on Dogs. Veterinary Parasitology, 201, 229-231.
[12]  Gassel, M., Wolf, C., Noack, S., Williams, H. and Ilg, T. (2014) The Novel Isoxazoline Ectoparasiticide Fluralaner: Selective Inhibition of Arthropod γ-Aminobutyric Acid- and l-Glutamate-Gated Chloride Channels and Insecticidal/Acaricidal Activity. Insect Biochemistry and Molecular Biology, 45, 111-124.
[13]  Lahm, G.P., Cordova, D., Barry, J.D., Pahutski, T.F., Smith, B.K., Long, J.K., Benner, E.A., Holyoke, C.W., Joraski, K., Xu, M., Schroeder, M.E., Wagerle, T., Mahaffey, M.J., Smith, R.M. and Tong, M.H. (2013) 4-Azolylphenyl Isoxazoline Insecticides Acting at the GABA Gated Chloride Channel. Bioorganic and Medicinal Chemistry Letters, 23, 3001-3006.
[14]  Shoop, W.L., Hartline, E.J., Gould, B.R., Waddell, M.E., McDowell, R.G., Kinney, J.B., Lahm, G.P., Long, J.K., Xu, M., Wagerle, T., Jones, G.S., Dietrich, R.F., Cordova, D., Schroeder, M.E., Rhoades, D.F., Benner, E.A. and Confalone, P.N. (2014) Discovery and Mode of Action of Afoxolaner, a New Isoxazoline Parasiticide for Dogs. Veterinary Parasitology, 201, 179-189.
[15]  EMA (2021) Guideline for the Testing and Evaluation of the Efficacy of Antiparasitic Substances for the Treatment and Prevention of Tick and Flea Infestation in Dogs and Cats. EMEA/CVMP/005/00-FINAL-Rev.1.
[16]  Halos, L., Baneth, G., Beugnet, F., Bowman, A. S., Chomel, B., Farkas, R., Franc, M., Guillot, J., Inokuma, H., Kaufman, R., Jongejan, F., Joachim, A., Otranto, D., Pfister, K., Pollmeier, M., Sainz, A. and Wall, R. (2012) Defining the Concept of “Tick Repellency” in Veterinary Medicine. Parasitology, 139, 419-423.
[17]  Marchiondo, A.A., Holdsworth, P.A., Fourie, L.J., Rugg, D., Hellmann, K., Snyder, D.E. and Dryden, M.W. (2013) World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) Second Edition: Guidelines for Evaluating the Efficacy of Parasiticides for the Treatment, Prevention and Control of Flea and Tick Infestations on Dogs and Cats. Veterinary Parasitology, 194, 84-97.
[18]  Pfister, K. and Armstrong, R. (2016) Systemically and Cutaneously Distributed Ectoparasiticides: A Review of the Efficacy against Ticks and Fleas on Dogs. Parasites and Vectors, 9, Article No. 436.
[19]  Six, R. H., Young, D. R., Myers, M. R. and Mahabir, S. P. (2016) Comparative Speed of Kill of Sarolaner (SimparicaTM) and Afoxolaner (NexGard®) against Induced Infestations of Ixodes scapularis on Dogs. Parasites and Vectors, 9, Article No. 79.
[20]  Fankhauser, R., Hamel, D., Dorr, P., Reinemeyer, C.R., Crafford, D., Bowman, D.D., Ulrich, M., Yoon, S. and Larsen, D.L. (2016) Efficacy of Oral Afoxolaner Plus Milbemycin Oxime Chewables against Induced Gastrointestinal Nematode Infections in Dogs. Veterinary Parasitology, 225, 117-122.
[21]  Hampel, V., Knaus, M., Schäfer, J., Beugnet, F. and Rehbein, S. (2018) Treatment of Canine Sarcoptic Mange with Afoxolaner (NexGard®) and Afoxolaner Plus Milbemycin Oxime (NexGard Spectra®) Chewable Tablets: Efficacy under Field Conditions in Portugal and Germany. Parasite, 25, Article No. 63.
[22]  Romero-Núñez, C., Bautista-Gómez, L.G., Sheinberg, G., Martín-Cordero, A., Flores-Ortega, A. and Heredia-Cárdenas, R. (2020) Efficacy of Afoxolaner Plus Milbemycin Oxime and Afoxolaner Alone as Treatment for Sarcoptic Mange in Naturally Infested Dogs. Canadian Journal of Veterinary Research, 84, 212-216.
[23]  Forster, S., Wiseman, S. and Snyder, D.E. (2021) Field Study to Investigate the Effectiveness and Safety of a Novel Orally Administered Combination Drug Product Containing Milbemycin Oxime and Lotilaner (Credelio® Plus) against Natural Flea and Tick Infestations on Dogs Presented as Veterinary Patients. Parasites and Vectors, 14, Article No. 299.
[24]  Packianathan, R., Hodge, A., Bruellke, N., Jackson, C. and Maeder, S. (2020) Efficacy of Combination Products Containing Sarolaner, Moxidectin and Pyrantel (Simparica TrioTM) or Afoxolaner and Milbemycin (NexGard Spectra®) against Induced Infestations of Ixodes holocyclus in Dogs. Parasites and Vectors, 13, Article No. 448.
[25]  Becskei, C., De Bock, F., Illambas, J., Mahabir, S.P., Farkas, R. and Six, R.H. (2016) Efficacy and Safety of a Novel Oral Isoxazoline, Sarolaner (SimparicaTM) in the Treatment of Naturally Occurring Flea and Tick Infestations in Dogs Presented as Veterinary Patients in Europe. Veterinary Parasitology, 222, 49-55.
[26]  Tinkruejeen, G., Meesaimongkon, P., Tangtrongsup, S., Thitaram, N., Srifawattana, N., Beugnet, F. and Tiwananthagorn, S. (2019) Comparative Efficacy of Afoxolaner and Ivermectin in Dogs Naturally Infested with Rhipicephalus sanguineus Sensu Lato: A Clinical Field Study Conducted in Thailand. Veterinary Parasitology: Regional Studies and Reports, 18, Article ID: 100340.
[27]  Beugnet, F., Liebenberg, J. and Halos, L. (2015) Comparative Efficacy of Two Oral Treatments for Dogs Containing Either Afoxolaner or Fluralaner against Rhipicephalus sanguineus Sensu Lato and Dermacentor reticulatus. Veterinary Parasitology, 209, 142-145.
[28]  Kunkle, B., Daly, S., Dumont, P., Drag, M. and Larsen, D. (2014) Assessment of the Efficacy of Orally Administered Afoxolaner against Rhipicephalus sanguineus Sensu Lato. Veterinary Parasitology, 201, 226-228.
[29]  Beugnet, F., Halos, L., Larsen, D., Labuschagné, M., Erasmus, H. and Fourie, J. (2014) The Ability of an Oral Formulation of Afoxolaner to Block the Transmission of Babesia canis by Dermacentor reticulatus Ticks to Dogs. Parasites and Vectors, 7, Article No. 283.
[30]  Geurden, T., Becskei, C., Six, R.H., Maeder, S., Latrofa, M.S., Otranto, D. and Farkas, R. (2018) Detection of Tick-Borne Pathogens in Ticks from Dogs and Cats in Different European Countries. Ticks and Tick-Borne Diseases, 9, 1431-1436.
[31]  Giannelli, A., Capelli, G., Joachim, A., Hinney, B., Losson, B., Kirkova, Z., René-Martellet, M., Papadopoulos, E., Farkas, R., Napoli, E., Brianti, E., Tamponi, C., Varcasia, A., Margarida, A., Madeira, L., Carvalho, D., Cardoso, L., Maia, C., Mircean, V., et al. (2017) Lungworms and Gastrointestinal Parasites of Domestic cats: A European Perspective. International Journal for Parasitology, 47, 517-528.
[32]  Patra, G., Sahara, A., Ghosh, S., Behera, P., Borthakur, S. K., Biswas, P., Debbarma, A. and Sahanawaz Alam, S. (2020) Prevalence of Tick-Borne Pathogens in Domestic Dogs in North-Eastern Region of India. Biological Rhythm Research, 51, 184-193.
[33]  Sheldon, I.M., Lewis, G.S., Leblanc, S. and Gilbert, R.O. (2006) Defining Postpartum Uterine Disease in Cattle. Theriogenology, 65, 1516-1530.
[34]  Baneth, G. (2014) Tick-Borne Infections of Animals and Humans: A Common Ground. International Journal for Parasitology, 44, 591-596.
[35]  Littman, M.P., Gerber, B., Goldstein, R.E., Labato, M.A., Lappin, M.R. and Moore, G.E. (2018) ACVIM Consensus Update on Lyme borreliosis in Dogs and Cats. Journal of Veterinary Internal Medicine, 32, 887-903.


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