Aim: This study aims to clarify the current state of advancement of knowledge and skills in the educational process from self-assessments by midwifery student labor and delivery assistance evaluation sheets. Method: Metrology is an observational and retrospective study. Self-assessments in labor and delivery assistance evaluation sheets of midwifery students registered in an advanced midwifery course at Nursing College A from Apr. 2017 to Mar. 2018 were analyzed retrospectively. The end-points were every score of six classifications: health checkups and assessments of women who give birth, preparations for labor and delivery assistance, delivery assistance in the parturient second stage, delivery assistance in the parturient third and fourth stages, midwifery process, and the training manner. Moreover, they also included the scores of 64 evaluation items belonging to any of the six classifications and a comprehensive evaluation score in which students judged the acquisition of all knowledge and skills of delivery assistance. In addition, although not on the evaluation sheet, the average score of all 64 evaluation items was also included. The evaluation criteria were five levels, from one to five. The end-points were examined by comparing the experiences of delivery assistances in two periods of <6 or 6 ≤ times. JMP16.2 was used. This study was approved by the institutional review board (IRB). Results: 80 among 91 evaluation sheets from eight out of nine students were analyzed. The average score of all 64 evaluation items, every score of the six classifications, and the comprehensive evaluation score was significantly elevated in the experience periods of 6 ≤ times (P < 0.001). Moreover, in all of the 63 detailed items except the item of being able to tell the adaptation of amniotomy, and to judge and carry it out at an appropriate time, all of the scores were significantly elevated (P < 0.05). In the experience periods of 6 ≤ times, the items with a median of two are “Being able to tell the adaptation of amniotomy, and to judge and carry it out at an appropriate time” and “Being able to assist with laceration (incision) sutures”. Conclusion: Training in adaptation and enforcement of amniotomy and assistance skills for lacerations sutures will be needed in future educational challenges. Self-assessment scores by midwifery students in labor and delivery assistance evaluation sheets were significantly elevated in the experience periods of 6 ≤
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