决明子蒽醌类提取物对肥胖小鼠肠道菌群的影响
Effects of Anthraquinone Extracts from Semen Cassiae on Intestinal Microflora of Obese Mice

目的：观察决明子提取物蒽醌类化合物对高脂饮食诱导的肥胖小鼠肠道菌群的影响。方法：ICR小鼠40只随机分为5组，即正常组、模型组、决明子正常剂量组、决明子低剂量组和决明子高剂量组。正常组给予生理盐水灌胃，其他组灌胃决明子蒽醌类提取物，四周后观察变化。生化法检测甘油三酯、总胆固醇、高密度脂蛋白、低密度脂蛋白；收集粪便提取DNA，采用荧光定量PCR法观察肠道菌群变化。结果：与正常对照组相比，高脂模型组的甘油三酯水平显著升高(P < 0.01)，HDL，LDL水平有所升高但未见明显变化；双歧杆菌，乳酸杆菌的数量降低(P < 0.05)，金黄色葡萄球菌和阴沟肠杆菌的数量升高(P < 0.05)。与高脂模型组相比，决明子蒽醌类物质药物干预组的TG水平明显下降(P < 0.05)，TC，HDL水平有所升高(P < 0.05)，LDL水平未见明显变化；双歧杆菌，乳酸杆菌的数量均出现了明显的升高(P < 0.05)，而金黄色葡萄球菌和阴沟肠杆菌的数量也有明显下降(P < 0.05)。结论：决明子蒽醌类物质治疗肥胖有一定的效果，其干预肥胖的药效机制可能与肠道中的双歧杆菌，乳酸杆菌的数量增加及金黄色葡萄球菌，阴沟肠杆菌的数量降低有关。
Objective: To observe the effects of anthraquinones extracted from cassia seed on intestinal microflora of obese mice induced by high fat diet. Methods: 40 ICR mice were randomly divided into 5 groups, namely normal group, model group, normal dose group, low dose group and high dose group of Cassia. The normal group was given normal saline intragastric administration, and the other groups were given anthraquinone extract of semen cassiae intragastric administration. The changes were observed after four weeks. Triglyceride, total cholesterol, high density lipoprotein and low density lipoprotein were detected by biochemical method. Feces were collected to extract DNA, and the changes of intestinal flora were observed by fluorescence quantitative PCR. Results: Compared with the normal control group, triglyceride levels in the high-fat model group were sig-nificantly higher (P < 0.01), HDL and LDL levels increased but no significant changes were observed; The number of bifidobacterium and lactobacillus decreased (P < 0.05), Staphylococcus aureus and Enterobacter cloacae increased (P < 0.05). Compared with the high-fat model group, the level of TG in the cassia anthraquinone drug intervention group significantly decreased (P < 0.05), TC and HDL levels increased (P < 0.05), there was no significant change in LDL level; The number of bifidobacterium and lactobacillus increased significantly (P < 0.05), while the number of Staphylo-coccus aureus and Enterobacter cloacae also decreased significantly (P < 0.05). Conclusion: The anthraquinones of cassia seed have certain effect on the treatment of obesity, and its pharmacodynamic mechanism may be related to the increase of the number of bifidobacterium and lactobacillus in intestinal tract and the decrease of the number of Staphylococcus aureus and Enterobacter cloacae.