应用RGB分形技术无损检测异叶茴芹、芹菜和香芹的药用成分及抗氧化活性
Nondestructive Evaluation of the Medicinal Components and Antioxidant Activities in the Pimpinella diversifolia, Apium graveolens and Petroselinum crispum by RGB Fractal Analysis

目的：比较异叶茴芹、芹菜和香芹三种伞形科植物茎、叶的药用成分及抗氧化活性，并应用RGB分形技术和数学建模无损检测其药用成分和抗氧化活性，为筛选药用成分含量高和抗氧化活性强的药用植物和药用部位提供理论依据。方法：用分光光度法测定异叶茴芹、芹菜和香芹茎、叶的总酚、总类黄酮、总可溶性糖、总香豆素、芹菜素的含量及DPPH自由基、ABTS自由基的清除活力；运用SPSS 20.0软件对结果进行相关性分析，并得到各药用部位、各药用成分的线性方程。结果：异叶茴芹茎、叶中各药用成分含量及抗氧化活性均显著高于其余两种伞形科植物；异叶茴芹叶的各药用成分及抗氧化活性显著高于茎；三种伞形科植物叶的R值、G值、B值均与其药用成分含量和抗氧化活性呈极显著负相关(P < 0.01)。结论：RGB分形技术可无损检测药用植物的药用成分及抗氧化活性；三种植物中，异叶茴芹的各药用成分及抗氧化活性最高，其叶的保健、药用价值更高，可广泛开发利用。
Objective: Comparing the medicinal components and antioxidant activities in the stems and leaves of the Pimpinella diversifolia, Apium graveolens and Petroselinum crispum, and using RGB fractal analysis and mathematical modeling to noninvasively evaluate the medicinal components and antioxidant activities, which provide a basis for screening the medicinal plants and medicinal parts with highly medicinal components and strongly antioxidant activities. Methods: The content of total phenol, total flavonoids, total soluble sugars, total coumarin and the scavenging activities of DPPH radical and ABTS radical in the stems and leaves of the Pimpinella diversifolia, A. graveolens and Petroselinum crispum were measured by spectrophotometry. SPSS 20.0 software was used to analyze the correlation of the results, and the linear equation of the medicinal components and medicinal parts was established. Results: The results showed that the content of medicinal components and antioxidant activities in the stems and leaves of Pimpinella diversifolia were significantly higher than those of the other two Umbelliferae plants, and the medicinal components and antioxidant activities of Pimpinella diversifolia’s leaves were significantly higher than its stems, and the RGB intensity values of the three Umbelliferae plants were significantly negatively correlated with the contents of medicinal components and antioxidant activities (P < 0.01), respectively. Conclusion: RGB fractal analysis can be applied to nondestructively detect the medicinal components and antioxidant activities of medicinal plants. Among the three plants, Pimpinella diversifolia has the highest medicinal components and antioxidant activities. Its leaves have higher health care and medicinal value than its stems, and may be widely exploited and utilized.