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油茶壳提取物对酪氨酸酶的抑制机理
Inhibitory Mechanism of Camellia oleifera Shell Extract on Tyrosinase

DOI: 10.12677/HJFNS.2022.112018, PP. 147-156

Keywords: 油茶壳,抑制,机理,酪氨酸酶
Camellia oleifera
, Inhibition, Mechanism, Tyrosinase

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Abstract:

目的:探究油茶壳提取物对酪氨酸酶的抑制作用,判断其抑制类型,初步探究其抑制机理,并对其活性成分进行测定。方法:分别以L-酪氨酸与L-多巴(L-DOPA)为底物,测定油茶壳提取物对酪氨酸酶单酚酶活性与二酚酶活性的抑制效果,Lineweaver-Burk双倒数作图法探究其抑制机理,并测定油茶壳提取物中的总多酚和黄酮含量。结果:油茶壳提取物对酪氨酸酶单酚酶和二酚酶的IC50分别为0.0711 mg/mL、0.2199 mg/mL,对酶底物络合物抑制常数KIS为10.7487 mg/mL。油茶壳提取物中总多酚含量为30.22% ± 1.02%,黄酮含量为13.52% ± 0.24%。结论:油茶壳提取物对酪氨酸酶单酚酶与二酚酶均具有较好抑制效果,总多酚、黄酮可能是油茶壳提取物发挥抑制酪氨酸酶活性作用的主要物质。
Objective: To explore the inhibitory effect of
Camellia oleifera shell extract on tyrosinase, to determine the type of inhibition, to preliminarily explore the inhibition mechanism, and to determine its active components. Method: Using L-tyrosine and L-DOPA as substrates, respectively, the inhibitory effects of Camellia oleifera shell extract on tyrosinase monophenolase activity and diphenolase activity were determined. The Lineweaver-Burk double-reciprocal plot method was used to explore the inhibition mechanism, and the contents of total polyphenols and flavonoids in Camellia oleifera shell extract were determined. Results: The IC50 of Camellia oleifera shell extract for tyrosinase monophenolase and diphenolase were 0.0711 mg/mL and 0.2199 mg/mL, respectively, and the KIS for enzyme-substrate complex was 10.7487 mg/mL. The total polyphenol content in Camellia oleifera shell extract was 30.22% ± 1.02%, and the flavonoid content was 13.52% ± 0.24%. Conclusion: Camellia oleifera shell extract has a good inhibitory effect on tyrosinase monophenolase and diphenolase. Total polyphenols and flavonoids may be the main substances of Camellia oleifera
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