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Botanical Research 2022
“桂柚1号”种子主要活性成分测定及其对糖尿病的初步药理作用研究
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Abstract:
以新选育的沙田柚新品种“桂柚1号”为试材，开展了“桂柚1号”种子主要活性成分的提取、测定，并以糖尿病重要靶点——钠–葡萄糖协同转运蛋白2 (SGLT-2)为目标开展了活性成分初步的抗糖尿病作用研究。结果表明，“桂柚1号”种子主要活性成分为柠檬苦素、柚皮苷和新橙皮苷；其中，新橙皮苷和柚皮苷具有相对较好的抑制SGLT-2活性的作用并在糖尿病小鼠模型上表现出了降血糖作用，降糖率分别为31.29%和26.32%。研究结果对“桂柚1号”综合开发利用具有重要意义。
The extraction and determination of the main active ingredient from the seeds of “Guiyou No.1”, a new variety of Shatian pomelo as the testing piece, were carried out. The antidiabetic effect of sodium glucose cotransporter 2 (SGLT-2) of the active ingredient, an important target of diabetes mellitus, was studied. The results showed that limonin, naringin and neohesperidin were the main active ingredient of “Guiyou No.1” seed. Among them, neohesperidin and naringin showed relatively good inhibitory effect on SGLT-2 activity, and showed hypoglycemic effect in diabetic mouse model, the hypoglycemic rate was 31.29% and 26.32%, respectively. The results are significant for the comprehensive development and utilization of “Guiyou No.1”.
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