本文研究了寒冷地区山区河流浮游植物生态化学计量学特征及元素间平衡关系。在2020年5月、2020年8月、2020年11月、2021年2月采集河北省张家口市崇礼区太子城河四个季度的浮游植物样品，测定碳(C)、氮(N)、磷(P)、硫(S)、氢(H)、铁(Fe)元素含量，分析其生态化学计量学特征及相关性。结果表明，浮游植物C、N、P、S、H、Fe元素含量分别为82.14 ± 32.12 g/kg、9.22 ± 3.5g/kg、1.46 ± 0.55 g/kg、1.96 ± 0.86 g/kg、2.36 ± 1.36 g/kg、12.64 ± 10.57 g/kg。整体上C、N、P元素含量相对稳定，S、H、Fe元素含量波动较大，Fe元素变异系数高达83.62%。太子城河浮游植物元素摩尔组成为C156.00N15.41S1.54H51.17Fe5.10P，与经典Redfield比值C106N16P相比存在差异。C元素占比较高表明太子城河浮游植物对C的需求高且固C能力强，N:P比值与前人研究结果吻合。太子城河浮游植物N:P比值为15.41，表明其生长受N、P共同限制。C、N、P元素含量间显著正相关，S、H、Fe之间无明显相关性；C:P与C:N、N:P间显著正相关，而C:N、N:P间以及H:S、Fe:S、H:Fe间相关性不强，表明浮游植物不同元素间耦联性不同，C、N、P作为浮游植物重要的营养元素相关性强。本文研究为深入理解寒冷山区河流浮游植物的生态化学计量学特征及元素平衡关系提供了基础。
This research performed an investigation on the phytoplankton ecological stoichiometry characteristics and the balance relationship between elements in a mountain river of cold region. The samples of phytoplankton of four seasons were collected in May 2020, August 2020, November 2020 and February 2021 from Taizicheng River in Chongli, Zhangjiakou City, Hebei Province, China. The contents of carbon (C), nitrogen (N), phosphorus (P), sulfur (S), hydrogen (H) and iron (Fe) were measured, and their ecological stoichiometry characteristics and correlation were analyzed. The results showed that, the contents of C, N, P, S, H and Fe of phytoplankton were 82.14 ± 32.12 g/kg, 9.22 ± 3.56 g/kg, 1.46 ± 0.55 g/kg, 1.96 ± 0.86 g/kg, 2.36 ± 1.36 g/kg and 12.64 ± 10.57 g/kg, respectively. Generally, the contents of C, N and P were relatively stable, while the contents of S, H and Fe fluctuated greatly, and the variation coefficient of Fe content reached as high as 83.62%. Moore of phytoplankton elements in Taizicheng River was C156.00N15.41S1.54H51.17Fe5.10P, which showed significant difference compared with the classical Redfield ratio C106N16P. The high proportion of element C indicated that phytoplankton in Taizicheng River had a high demand for C and a strong ability to consolidate C, and the N:P ratio was consistent with the results of previous studies. The ratio of N:P was 15.41, suggesting that the growth of phytoplankton in Taizicheng River was restricted by both N and P. The contents of C, N and P were positively correlated, while S, H and Fe had no significant correlation. C:P was significantly positively correlated with C:N and N:P, while C:N, N:P, H:S, Fe:S, and H:Fe were not strongly correlated, indicating that coupling
References
[1]
Moreno, A.R. and Martiny, A.C. (2018) Ecological Stoichiometry of Ocean Plankton. Annual Review of Marine Science, 10, 43-69. https://doi.org/10.1146/annurev-marine-121916-063126
[2]
Urabe, J., Nakanishi, M. and Kawabata, K. (1995) Contribution of Metazoan Plankton to the Cycling of N and P in Lake Biwa. Limnology and Oceanography, 40, 232-242.
[3]
Maklno, W., Coiner, J.B. and Stemer, R.W. (2003) Are Bacteria More like Animals than Plants? Growth Rate and Resource Dependence of Bacterial C:N:P Stoichiometry. Functional Ecology, 17, 121-130.
https://doi.org/10.1046/j.1365-2435.2003.00712.x
[4]
Stelzer, R.S. and Lamberti, G.A. (2002) Ecological Stoi-chiometry in Running Waters: Periphyton Chemical Composition and Snail Growth. Ecology, 83, 1039-1051. https://doi.org/10.1890/0012-9658(2002)083[1039:ESIRWP]2.0.CO;2
[5]
Elser, J. (2006) Biological Stoichi-ometry: A Chemical Bridge between Ecosystem Ecology and Evolutionary Biology. American Naturalist, 168, S25-S35. https://doi.org/10.1086/509048
[6]
Redfield, A.C. (1958) The Biological Control of Chemical Factors in the Environment. American Scientist, 46, 205-211.
[7]
Elser, J.J., Elser, M.M., MacKay, N.A., et al. (1988) Zoo-plankton-Mediated Transitions between N and P Limited Algal Growth. Limnology and Oceanography, 33, 1-14. https://doi.org/10.4319/lo.1988.33.1.0001
[8]
Downing, J.A. (1997) Marine Nitrogen: Phosphorus Stoichiometry and the Global N:P Cycle. Biogeochemistry, 37, 237-252. https://doi.org/10.1023/A:1005712322036
Anderson, L.A. and Sarmiento, J.L. (1994) Redfield Ratios of Remineralization Determined by Nutrient Data Analysis. Global Biogeochemical Cycles, 8, 65-88. https://doi.org/10.1029/93GB03318
Berger, S.A., Diehl, S. and Kunz, T.J. (2006) Light Supply, Plankton Biomass, and Seston Stoichiometry in a Gradient of Lake Mixing Depths. Limnology and Oceanography, 51, 1898-1905. https://doi.org/10.4319/lo.2006.51.4.1898
Geider, R.J. and Laroche, J. (1994) The Role of Iron in Phytoplankton Photosynthesis, and the Potential for Iron-Limitation of Primary Productivity in the Sea. Photosynthesis Research, 39, 275-301.
https://doi.org/10.1007/BF00014588
