以高嘌呤饮食评估罗伊氏乳杆菌Lactobacillus reuteri GKR1之降尿酸效果
Evaluation of Lactobacillus reuteri GKR1 on Lowering Uric Acid with High Purine Diet

DOI: 10.12677/HJFNS.2022.111007, PP. 56-63

Keywords: Lactobacillus reuteri GKR1，益生菌，尿酸，黄嘌呤氧化酶，高嘌呤饮食
Lactobacillus reuteri GKR1, Probiotics, Uric Acid, Xanthine Oxidase, High-Purine Diet

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Abstract:

近年来，高尿酸血症的盛行率居高不下，高嘌呤饮食为引起高尿酸血症之重要因素，高尿酸血症会增加罹患心血管疾病、糖尿病、肾脏病功能异常、高血压等疾病的机率，而降尿酸药物具有副作用，因此需开发替代治疗之策略。本试验先以体外试验筛选具有降尿酸潜力菌株，发现效果最佳菌株为Lactobacillus reuteri GKR1，再以高嘌呤饮食饲料评估Lactobacillus reuteri GKR1于小鼠之降尿酸效果，试验将ICR小鼠分为5组：正常对照组、负对照组、阳性对照组、低剂量GKR1组及高剂量GKR1组，连续14天将试验物质经管喂投予小鼠，在试验第7及第14天收集各组小鼠血清，分析尿酸(Uric acid)、尿素氮(BUN)与肌肝酸(Creatinine)含量，在试验第14天采集小鼠肝脏分析黄嘌呤氧化酶活性。实验结果显示，低、高剂量组GKR1可降低血清中尿酸、尿素氮含量，高剂量GKR1组显着减少肝脏中黄嘌呤氧化酶活性，综合以上结果，GKR1具有开发为降尿酸保健食品之潜力。
In recent years, the prevalence of hyperuricemia has remained high. High-purine diet is an important factor in causing hyperuricemia. Hyperuricemia will increase the risk of cardiovascular disease, diabetes, abnormal kidney disease, hypertension and other diseases. However, uric acid-lowering drugs have side effects, so it is necessary to develop alternative treatments. In this experiment, we first screened lactobacillus strains with uric acid-lowering potential by in vitro tests, and found that the best strain was Lactobacillus reuteri GKR1. Then, the high-purine diet was used to evaluate the uric acid-lowering effect of Lactobacillus reuteri GKR1 in mice. The test divided ICR mice into 5 groups: normal control group, negative control group, positive control group, low-dose GKR1 group and high-dose GKR1 group. The test substance was administered to the mice via tube for 14 consecutive days, and the serum of each group was collected on the 7th and 14th days of the test analyze the content of uric acid, urea nitrogen (BUN) and Creatinine, and collect and analyze the xanthine oxidase activity in mouse liver on the 14th day of the experiment. Experimental results show that low and high dose GKR1 can reduce serum uric acid and urea nitrogen content, and high dose GKR1 group significantly reduces xanthine oxidase activity in the liver. Based on the above results, GKR1 has the potential to be developed as a healthy food for lowering uric acid.

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