For identification of transgenic DNA of Arabidopsis plant material, the followed methods are transformation and growing of seeds
after bleaching them for sterilization should
be performed then using polymerase chain reaction (PCR) for amplification the gene before making gel
electrophoresis to be ready for southern blot analysis, this method is
possible because the length of the given primers, there are slightly
modifications in the methods as ligating PCR technique developed for
amplification of the unknown DNA after extracting it to determine its
transgenicity. The methods were specific and reproducible for many different
plants from transgenic and non-transgenic cell lines. Furthermore, the number
of products of PCR result can be considered as a good estimation of transgenic
DNA, during comparison to southern blot analysis, the results obtained by the
PCR give information about DNA of Arabidopsis, applications of the
approach to Arabidopsis plants after transformation with an Agrobacterium-mediated transformation
will be described.
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