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筛选与评估减缓肠道发炎功效之益生菌
Screening and Evaluation of Probiotics for Reducing Intestinal Inflammation

DOI: 10.12677/HJFNS.2022.111006, PP. 44-55

Keywords: 乳酸片球菌Pediococcus acidilactici GKA4，戊醣片球菌Pediococcus pentosaceus GKP4，肠道发炎， 葡聚糖硫酸，益生菌，肠胃道保护
Pediococcus acidilactici GKA4, Pediococcus pentosaceus GKP4, Intestinal Inflammation, Dextran Sulfate Sodium (DSS), Probiotics, Gastrointestinal Tract Protection

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Abstract:

本篇利用葡聚糖硫酸(Dextran Sulfate Sodium, DSS)诱发小鼠肠道发炎的模式来筛选与评估益生菌在减缓肠道发炎与损伤的效果。实验将8周龄的BALB/c小鼠分为六组(n = 10)：正常对照组(无DSS诱导)、负对照组(DSS诱导)、片球菌GKA4组(DSS + GKA4)、片球菌GKP4组(DSS + GKP4)、乳酸菌GKR1组(DSS + GKR1)、以及乳酸菌GK4组(DSS + GK4)。连续14天以相当于成人一天摄取1克的剂量管喂小鼠益生菌，并于第7天到第14天的饮水中加入3.5% DSS，藉以诱发肠道发炎。实验结果显示给予3.5% DSS后，片球菌GKA4组及GKP4组与负对照组相比下，其对于体重减轻、结肠长度、疾病活动指数等指标均有明显改善。此外，亦可降低血清中促炎细胞激素IL-1β、IL-6及TNF-α含量，藉此减缓由DSS引发的肠道发炎反应。综观上述结果，片球菌P. acidilactici GKA4与P. pentosaceus GKP4具有应用于肠道保护的益生菌潜力。
In this study, we applied Dextran Sulfate Sodium (DSS)-induced enteritis in mice to evaluate the effects of probiotics on reducing intestinal inflammation and damage. The 8-week-old BALB/c mice were divided into six groups (n = 10): normal group (without DSS), negative control group (DSS-induced), probiotic Pediococcus acidilactici GKA4 group (DSS + GKA4), Pediococcus pentosaceus GKP4 group (DSS + GKP4), Lactobacillus reuteri GKR1 group (DSS + GKR1), and GK4 group (DSS + GK4). Mice were given tested bacteria at an equivalent to 1 gram of adult daily intake for 14 consecutive days. Then 3.5% DSS was added to drinking water from day 7th to day 14th to induce intestinal damage. The results showed that the Pediococcus GKA4 group and GKP4 group were significantly improved in weight loss, colon length, disease activity index, and other indicators when compared with the negative control group. In addition, both Pediococcus groups reduced the levels of pro-inflammatory cytokines IL-1β,

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