本实验通过固体发酵，比较锌、锰、叶黄素、维生素D3、β-胡萝卜素、叶酸等几种微量营养素在蝉花子实体中的富集效果及其对生长的影响。在罐头瓶的小麦培养基中直接添加锌、锰、铬、叶黄素、维生素D3、β-胡萝卜素、叶酸等原料，发现最后培养所得蝉花子实体中，锌、锰、叶酸均有较好的富集效果，在0.25%添加量下，蝉花子实体中的锌含量由3.44 mg/100g升高至13.2 mg/100g，锰含量由0.91 mg/100g升高至10.3 mg/100g，叶酸含量由107 μg/100g升高至493 μg/100g，但锰原料组蝉花子实体生物量显著降低。进一步将固体培养扩大至培养盒，对锌、叶酸的添加浓度进行筛选，随着浓度上升，锌添加量0.9%时，在蝉花子实体中的富集量增加最快，超过0.9%，富集量的增速减缓，添加量达到1.5%，子实体生物量显著降低；叶酸添加量1.2%时，在蝉花子实体中富集量增加最快，而添加量达到1.5%，子实体生物量同样显著降低。
This article compares Zinc,
manganese, lutein, vitamin D3 β-Enrichment
effects of carotene, folic acid and other micronutrients in cicada flower
fruiting body and their effects on growth, by solid fermentation. When the
culturing started in cans, we found that Zinc, Manganese and Folic acid were
well enriched in the cicada flower fruiting body, after we directly added Zinc,
Manganese, Chromium, Lutein, Vitamin D3, β-Carotene and Folic acid raw materials.
With the addition of 0.25%, the content of Zinc in cicada flower
fruiting body increased from 3.44 mg/100g to 13.2 mg/100g, the content of Manganese
increased from 0.91 mg/100g to 10.3 mg/100g, and the content of Folic acid
increased from 107 μg/100g to 493 μg/100g, but the biomass of cicada flower
fruit body in Manganese raw material group decreased significantly. Then we
further expanded the solid culture to the culture box to screen the added
concentrations of zinc and folic acid. With the increase of Zinc concentration,
the enrichment in cicada flower fruiting body increased fastest when the
addition of zinc was 0.9%, more than 0.9%, the growth rate of enrichment slowed
down, the addition reached 1.5%, and the biomass of cicada flower fruiting body
decreased significantly; when the amount of folic acid was 1.2%, the enrichment
increased the fastest in cicada flower fruiting body, and when the amount of
folic acid was 1.5%, the biomass of cicada flower fruiting body also decreased
significantly.
