NaNO₂-Al(NO₃)₃-NaOH比色法测定乳苣不同部位总黄酮含量研究
Study on the NaNO₂-Al(NO₃)₃-NaOH Colorimetric Method for the Determination of Total Flavonoids in Different Parts of Mulgedium tataricum L.

DOI: 10.12677/HJFNS.2022.111005, PP. 36-43

Keywords: 乳苣，不同部位，总黄酮，NaNO₂-Al(NO₃)₃-NaOH比色法，含量测定
Mulgedium tataricum, Different Parts, Total Flavonoids, NaNO₂-Al(NO₃)₃-NaOH Colorimetric Method, Content Determination

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Abstract:

建立NaNO₂-Al(NO₃)₃-NaOH比色法，测定乳苣不同部位及其全草总黄酮含量。一定量样品液加5% NaNO2溶液2.00 ml，摇匀，放置6 min，10% Al(NO₃)₃溶液2.00 ml，摇匀，放置6 min，加4% NaOH溶液12.00 ml，蒸馏水定容至刻度，摇匀，静置15 min，于522 nm下测定吸光度，黄酮含量在0.0080~0.0720 mg/ml范围内与吸光度具有良好的线性关系。精密度和稳定性试验RSD分别为1.5%和2.9%，平均回收率93.61%，有较好的的精密度和准确度。本试验测得乳苣根、茎、叶及全草总黄酮提取率依次为：叶(105.4 mg/g) > 全草(77.63 mg/g) > 茎(12.60 mg/g) > 根(11.64 mg/g)，由此可知乳苣总黄酮主要集中在地上部分。
A NaNO₂-Al(NO₃)₃-NaOH colorimetric method was established to determine the total flavonoids content in different parts of Mulgedium tataricum L and its whole grass. A certain amount of sample solution was added with 2.00 ml of 5% NaNO₂ solution, shaken, and placed for 6 minutes, added with 12.00 ml of 4% NaOH solution, and distilled water to determine Volume up to the mark, shake well, stand for 15 min, measure the absorbance at 522 nm, the flavonoid content in the range of 0.0080~0.0720 mg/ml has a good linear relationship with the absorbance. The RSD of the precision and stability tests were 1.5% and 2.9%, respectively, and the average recovery rate was 93.61%, with good precision and accuracy. In this experiment, the extraction rate of total flavonoids from the roots, stems, leaves, and whole plants of Nicotiana officinalis was as follows: leaves (105.4 mg/g) > whole plants (77.63 mg/g) > stems (12.60 mg/g) > roots (11.64 mg/g), it can be seen that the total flavonoids of Mulgedium tataricum L. are mainly concentrated in the above-ground part.

References

[1]	中国科学院中国植物志编写委员会. 中国植物志第 80 卷第一分册[M]. 北京: 科学出版社, 1997: 75-76.
[2]	高义霞, 景红艳, 姜祖君, 等. 响应面分析法优化乳苣总黄酮提取工艺的研究[J]. 中药材, 2010, 33(4): 621-624.
[3]	周向军, 高义霞, 李娟娟, 等. 乳苣多酚提取工艺及抗氧化研究[J]. 中国酿造, 2011(9): 118-121.
[4]	Wang, X.-X., Lin, C.-J. and Jia, Z.-J. (2006) Triterpenoids and Sesquiterpenes from Mulgedium tataricum. Planta Medica, 72, 764-767. https://doi.org/10.1055/s-2006-941508
[5]	何宝佳, 陶浩楠, 魏蔚, 等. 微波萃取技术提取败酱草中总黄酮的应用研究[J]. 唐山师范学院学报, 2016, 38(5): 44-46.
[6]	王雪, 刘景楠, 夏顺利, 等. 中药总黄酮抗肿瘤活性及机制的研究现状[J]. 中国临床药理学杂志, 2021, 37(4): 484-486+496.
[7]	高义霞, 史陇陇, 王倩宁, 等. 不同溶剂乳苣萃取物对金黄色葡萄球菌抑制作用研究[J]. 天水师范学院学报, 2017, 37(5): 30-33.
[8]	李龙梅, 牛俊美, 铁英, 等. 不同生长期乳苣营养器官矿质营养元素含量的动态变化[J]. 中国瓜菜, 2020, 33(8): 40-44.
[9]	严赞开. 紫外分光光度法测定植物黄酮含量的方法[J]. 食品研究与开发, 2007, 28(9): 164-165.
[10]	马陶陶, 张群林, 李俊. 中药总黄酮的含量测定方法[J]. 安徽医药, 2007, 11(11): 1030-1032.
[11]	林勇, 朱良辉, 吴永忠. 比色法测定不同产地乌饭叶总黄酮含量[J]. 时珍国医国药, 1999, 10(2): 97.
[12]	景仁志, 陈波, 葛绍荣, 等. 薄层扫描法测定苦荞叶中芦丁的含量[J]. 四川大学学报(自然科学版), 1997, 36(4): 877-879.
[13]	李吉来, 于留荣. 薄层扫描法测定银杏叶中总黄酮醇甙的含量[J]. 中国中药杂志, 1996, 21(2): 106-108.
[14]	张廷之, 侯镜德, 徐秀珠. 反相高效液相色谱法测定毛竹叶中总黄酮[J]. 理化检验-化学分册, 2001, 37(3): 117-118.
[15]	王学军, 许振良, 赵锁奇. 银杏叶提取物中槲皮素和芦丁的超临界流体色谱法测定[J]. 中国医药工业杂志, 2005, 36(7): 415-417.
[16]	孙莲, 孟磊, 陈坚, 等. 毛细管电泳法测定桑叶中的黄酮类成分——芦丁和槲皮素[J]. 色谱, 2001, 19(5): 395-397.
[17]	楚红英, 李瑜, 李华北, 等. 苦菜中总黄酮的提取与含量测定[J]. 安徽农业科学, 2012, 40(24): 12017-12019.

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