菟丝子属六种杂草籽DNA条形码鉴定研究
Identification of DNA Barcodes in Six Weed Species of Cuscuta L.

DOI: 10.12677/HJAS.2021.1112153, PP. 1118-1123

Keywords: 菟丝子属，杂草种子，DNA条形码，ITS2，rbcL
Cuscuta L., Weed Seeds, DNA Barcode, ITS2, rbcL

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Abstract:

本研究通过比较用于植物鉴定的DNA条形码序列ITS1、ITS2、rbcL、matK在菟丝子属(Cuscuta L.) 6个种的杂草籽的种间差异，分析研究菟丝子属DNA条形码鉴定的主要特征。通过对样品进行DNA提取、扩增、测序，利用NCBI中的Nucleotide BLAST、邻接法(Neighbor-Joining)进行鉴定分析。经综合比较可以看出利用DNA条形码能够准确地鉴别菟丝子属杂草，其中ITS2、rbcL序列的鉴定成功率较高，适合作为鉴别菟丝子属杂草籽的条形码序列。本研究结果以期为DNA条形码技术在菟丝子属杂草籽的筛选鉴定中提供依据。
In this study, four DNA barcode fragments, ITS1, ITS2, rbcL and matK were used for plant identification in six species of Cuscuta L. to analyze the main characteristics of DNA barcoding identification of Cuscuta L.. Nucleotide BLAST and neighbor-joining in NCBI were used for DNA extraction, amplification and sequencing. Through comprehensive comparison, it can be seen that DNA barcoding can be used to identify weeds of Cuscuta accurately, and ITS2 and rbcL sequences have a higher success rate of identification, which is suitable for identifying Cuscuta L. seeds. The results are designed to establish the DNA barcode technique in the identification of Cuscuta L. seeds.

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