Objective: Pre-, pro- and postbiotics are becoming more prevalent as ingredients in cosmetic and personal care products. A novel triple biotic technology has been developed and investigated for its impact on skin flora and skin barrier properties. Methods: Growth inhibition/promotion assay was performed to determine the effect on skin bacteria growth, using Escherichia coli, Corynebacterium striatum, Staphylococcus aureus, and Staphylococcus epidermidis. A skin penetration assay and skin barrier biomarker measurements were performed using an ex vivo human skin explant model. The triple-biotic complex of inulin, 2-butyloctanol, and a biomimic blend of postbiotics was tested individually as well as part of cosmetic formulations. Results: The triple-biotic technology, either as individual components or in a cosmetic formulation, inhibited the growth of undesirable bacteria, in most cases. On the other hand, the growth of desirable bacteria was either promoted or maintained. The cosmetic formulations with the triple-biotic technology demonstrated an enhanced skin barrier and an increase in skin barrier biomarkers. Conclusion: A novel triple-biotic technology has been developed and shown to deliver a strong prebiotic effect with demonstrable benefits on bacterial growth, skin barrier properties, and the production of skin barrier biomarkers. This study indicates that triple-biotic technology can be used as a desirable prebiotic ingredient in personal care products to provide skin health benefits.
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