氢气吸入对传染性单核细胞增多症淋巴细胞亚群的影响
Effect of Hydrogen Gas Inhalation on T Lymphocyte Subsets in Infectious Mononucleosis

目的：探讨高浓度氢气(66.7%)吸入对IM患儿临床症状及免疫状态的影响。方法：选取传染性单核细胞增多症患儿60例，年龄1~10岁，采用随机数字表达分为两组：氢气吸入组和常规治疗组，每组各30例；常规治疗组给予阿昔洛韦抗病毒及对症常规治疗，氢气吸入组在常规治疗组治疗方案的基础上加用氢气吸入(每天给予含66.7%氢气和33.3%氧气的混合气体吸入60分钟)，疗程10天。观察患儿治疗前及治疗后临床症状及外周血淋巴细胞亚群水平变化。结果：经治疗后，氢气吸入组和常规治疗组，两组患儿均表现为：CD8+细胞水平降低，CD4+细胞水平增高，CD4+/CD8+明显升高，治疗前后比较差异有统计学意义(均P < 0.05)；治疗后，比较常规治疗组，氢气吸入组的CD8+细胞水平降幅比率，CD4+细胞水平增幅比率及CD4+/CD8+升幅比率更大，差异显著(均P < 0.05)。结论：阿昔洛韦联合氢气吸入治疗IM，对于由病毒感染应激造成的CD4+、CD8+T细胞紊乱，具有更好的恢复效果，优于单纯使用阿昔洛韦，氢氧2:1吸入可促进T细胞免疫功能的尽快恢复，其机制可能与调控炎症反应有关。
Objective: To investigate the effect of high concentration of hydrogen gas (66.7%) inhalation on clinical symptoms and the immune status of children with IM. Methods: 60 patients with IM (aged 1~10 years old) were selected, and randomly divided into routine treatment group and hydrogen inhalation group, with 30 cases in each group. The routine treatment group was treated with acyclovir for antivirus and symptomatic treatment for 10 days. The hydrogen inhalation group was given acyclovir, and mixed gas containing 66.7% hydrogen and 33.3% oxygen inhalation for 60 min plus the same treatment as in routine treatment group. The changes in peripheral blood T lymphocyte subsets and the clinical effects were compared between the two groups．Results: After treatment, both the hydrogen inhalation group and the routine treatment group showed decreased CD8+ cell level, increased CD4+ cell level, and increased CD4+/CD8+, with statistically significant differences compared to those before treatment (P < 0.05); the reduction ratio of CD8+ cell level, the increase ratio of CD4+ cell level and the increase ratio of CD4+/CD8+ in the hydrogen inhalation group were significantly higher than those in the conventional treatment group (P < 0.05). Conclusion: Acyclovir combined with hydrogen inhalation has a better recovery effect on CD4+ and CD8+ T cell disorders caused by the stress of viral infection in the treatment of IM, which is better than acyclovir alone. High concentration of hydrogen inhalation may significantly promote the recovery of T cell immune function, the mechanism of which may be associated with the regulation of inflammatory response.