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两种软件测量黄斑中心凹下脉络膜厚度的比较
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Abstract:
目的：评估Image J软件和Cirrus-HD OCT内置软件测量脉络膜厚度的一致性和重复性。方法：研究纳入102人204只眼，采用增强成像技术(enhanced depth imaging, EDI)，以6.0 mm线段对黄斑部水平及垂直方向进行五线高清扫描。使用EDI技术获得脉络膜全层图像后应用Image J软件及Cirrus-HD OCT内置软件分别测量黄斑中心凹下脉络膜厚度(subfoveal choroidal thickness, SFCT)后，进行Bland-Altman分析、运用组内相关系数(intraclass correlation coefficient, ICC)及重复性系数(repeatability coefficient, Rc)比较两种软件测量脉络膜厚度结果的一致性和重复性。结果：Image J软件及Cirrus-HD OCT内置测量软件测定SFCT的结果分别为224.55 ± 42.91 μm和222.78 ± 43.80 μm；两组方法学比较ICC值为0.985 (P < 0.05)；两种软件的一致性界限(limits of agreement, LoA)为？13.956~17.505 μm。Cirrus-HD OCT内置测量软件的重复性系数为11.2764 μm；Image J软件重复性系数为9.0694 μm，低于前者。结论：两种软件对于黄斑中心凹下脉络膜厚度的测量结果有很好的一致性，但Image J软件的重复性优于Cirrus-HD OCT内置测量软件，因此，在未来的临床应用或研究中，建议使用Image J软件测量脉络膜厚度。
Objective: To investigate the agreement and repeatability of choroidal thickness measurements determined by Image J software and Zeiss Cirrus HD-OCT intrinsic software. Methods: One hundred and two study participants (204 eyes) were enrolled in this research. Five line scanning by Cirrus-HD OCT with enhanced depth imaging (EDI) was performed on the area of fovea at the vertical and horizontal azimuth with scanning length of 6.0 mm. The subfoveal choroidal thickness was measured manually by Image J software and Cirrus-HD OCT intrinsic software. The agreement and repeatability of two kinds of software were described by Bland-Altman analysis, intraclass correlation coefficient (ICC) and repeatability coefficient (Rc). Results: The mean (±s) subfoveal choroidal thickness was 224.55 ± 42.91 μm for Image J software and 222.78 ± 43.80 μm for the Cirrus-HD OCT intrinsic software. The ICC of two kinds of software was 0.985 (P < 0.05), and the limits of agreement (LoA) were ？13.956~17.505 μm. As compared with Cirrus-HD OCT software (11.2764 μm), the repeatability coefficient is lower calculated by Image J software (9.0694 μm). Conclusion: There was a good agreement between Image J and Cirrus-HD OCT software. The repeatability of measurement implied by Image J software was better than the Cirrus-HD OCT software. Thus, the Image J software should be used for measuring the choroidal thickness in future study.
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