安徽省水稻纹枯病菌对吡唑醚菌酯的敏感性及其与不同杀菌剂间的交互抗性
Sensitivity of Rice Sheath Blight Pathogen Rhizoctonia solani to Pyraclostrobin and Cross-Resistance against Diverse Fungicides in Anhui Province

DOI: 10.12677/HJAS.2021.1111147, PP. 1079-1085

Keywords: 水稻纹枯病菌，吡唑醚菌酯，敏感基线，交互抗性
Rhizoctonia solani, Pyraclostrobin, Baseline Sensitivity, Cross-Resistance

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Abstract:

采用菌丝生长速率法测定了该省15个地区的94株水稻纹枯病菌菌株对吡唑醚菌酯的敏感性，以明确安徽省水稻纹枯病菌Rhizoctonia solani对吡唑醚菌酯的敏感性及其与不同杀菌剂间的交互抗性，结果表明，供试94株水稻纹枯病菌菌株对吡唑醚菌酯具有较高的敏感性，所有菌株的有效抑制中浓度EC50在0.0409~1.2266 μg/mL之间，平均值为0.2721 μg/mL，其敏感性频率呈连续单峰曲线分布，符合正态分布，因此可将EC50平均值0.2721 μg/mL作为安徽省水稻纹枯病菌对吡唑醚菌酯的敏感基线；15个地区的菌株群体对吡唑醚菌酯的敏感性差异显著，同一地区的菌株个体间差异较大；吡唑醚菌酯与噻呋酰胺、丙硫菌唑及井冈霉素之间均不存在交互抗性。
To determine the sensitivity of rice sheath blight pathogen Rhizoctonia solani to Pyraclostrobin, and evaluate the cross-resistance between Pyraclostrobin and other fungicides, a total of 94 R. solani isolates collected from 15 regions in Anhui Province were used for testing their sensitivity to Pyraclostrobin using mycelial growth method. The tested isolates were highly sensitive to Pyraclostrobin, and the EC50 of these isolates to Pyraclostrobin were in the range of 0.0409~1.2266 μg/mL, with the mean value of 0.2721 μg/mL. The frequency curve of Pyraclostrobin was continuous and unimodal, and followed the normal distribution. Hence, the mean EC50 of 0.2721 μg/mL could be served as the baseline sensitivity of R. solani to Pyraclostrobin in Anhui Province. There was significant difference in the sensitivity to Pyraclostrobin among R. solani populations from 15 regions; there was an obvious difference in the sensitivity to Pyraclostrobin among individual isolate from same regions in Anhui Province. There was no notable evidence of positive cross-resistance between Pyraclostrobin and Jinggangmycin, thifluzamide and prothioconazole.

References

[1]	Ghosh, S., Mirza, N., Kanwar, P., et al. (2019) Genome Analysis Provides Insight about Pathogenesis of Indian Strains of Rhizoctonia solani in Rice. Functional & Integrative Genomics, 19, 799-810. https://doi.org/10.1007/s10142-019-00687-y
[2]	Savary, S. (1995) Direct and Indirect Effects of Nitrogen Supply and Disease Source Structure on Rice Sheath Blight Spread. Phytopathology, 85, 959-965. https://doi.org/10.1094/Phyto-85-959
[3]	Slaton, N.A., Cartwright, R.D., Meng, J., et al. (2003) Sheath Blight Severity and Rice Yield as Affected by Nitrogen Fertilizer Rate, Application Method, and Fungicide. Agronomy Journal, 95, 1489-1496. https://doi.org/10.2134/agronj2003.1489
[4]	常望霓. 水稻纹枯病防治药剂的筛选及田间防效[D]: [硕士学位论文]. 长沙: 湖南农业大学, 2011.
[5]	Mu, W., Wang, Z., Bi, Y., et al. (2017) Sensitivity Determination and Re-sistance Risk Assessment of Rhizoctonia solani to SDHI Fungicide Thifluzamide. Annals of Applied Biology, 170, 240-250. https://doi.org/10.1111/aab.12334
[6]	齐永志, 李海燕, 苏媛, 等. 小麦纹枯病菌对噻呋酰胺的敏感性及抗药性突变体的主要生物学性状[J]. 农药学学报, 2014, 16(3): 271-280.
[7]	梁梦琦. 长江中下游稻区稻瘟病菌对稻瘟灵和吡唑醚菌酯的抗性监测[D]: [硕士学位论文]. 北京: 中国农业科学院, 2018.
[8]	杨丽娟, 柏亚罗. 甲氧基丙烯酸酯类杀菌剂——吡唑醚菌酯[J]. 现代农药, 2012, 11(4): 46-50+56.
[9]	刘世江, 丁怡, 赵琪君, 等. 贵州省水稻纹枯病菌对丙环唑和吡唑醚菌酯的敏感性[J]. 福建农业学报, 2019, 34(11): 1294-1301.
[10]	毕秋艳, 马志强, 韩秀英, 等. 不同机制杀菌剂对小麦白粉病的敏感性及与三唑酮的交互抗性[J]. 植物保护学报, 2017, 44(2): 331-336.
[11]	Russell, P.E. (2004) Sensitivity Baselines in Fungicide Resistance Research and Manage-ment. FRAC Monograph, No. 3, RAC, Brussels, Belgium: AIMPRINT.
[12]	Zhou, Y.X., Chen, L., Hu, J., Liu, P.F., Zhang, Y., Meng, Q.X., Li, B., Si, N.G., Liu, C.L. and Liu, X.L. (2016) Aseline Sensitivity of Natural Population and Resistance Risk of Peronophythora litchii to Four Novel QoI Fungicides. European Journal of Plant Pathology, 146, 71-83. https://doi.org/10.1007/s10658-016-0893-y
[13]	孙雪. 水稻纹枯病的防治技术研究[D]: [硕士学位论文]. 长春: 吉林农业大学, 2015.

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