This study assessed the effect of processing method on the carotenoid profile of oil from three varieties of Nigerian oil palm fruits (Elaise guinensis). Specific varieties of the Elaise guinensis, which are the dura, pesifera and tenera were obtained from National Institute for Oil Palm Research (NIFOR) in Edo state, Nigeria. The samples were divided into two and processed using two different methods as commonly practiced in south-eastern Nigeria, the hot and cold processes. The oil extracted from each of the processes was then analyzed for oil characteristics using standard analytical methods while the carotenoid profile was analyzed using HPLC C-21 column. The results generated were subjected to the one-way analysis of variance (ANOVA). The results of carotenoid constituent identified include: lutein, neurosporene (trans), neurosporene (cis), α-Zeacarotene(cis), α-Zeacarotene(trans), phytoene, phytofluene, β-zeacarotene, 13 and 13’ cis α-carotene, 13 cis β-carotene, trans α-carotene, 9 cis α-carotene, trans β-carotene, αcarotene a(cis), α-carotene b(cis), α-carotene (trans), γ-carotene (trans), γ-carotene b(cis), lycopene (cis) and lycopene (trans). The results of physicochemical characteristics of the oil samples extracted range from 0.922 ± 0.004 - 0.916 ± 0.001 for specific gravity (SG), 8.10 ± 0.17 - 4.88 ± 0.04 mg KOH/g for acid value, 4.29% ± 0.02% - 2.44% ± 0.02% for free fatty acid value (FFA), 6.00 ± 0.21 to 204.67 ± 0.98 mgKOH/g for saponification value and 9.53 ± 0.23 - 5.25 ± 0.33 mEq/kg; for peroxide value while the carotenoids values were between 53.735 ± 0.10 and 123.389 ± 0.20 mg/100g. From the result, we can observe that the main constituent of the palm oil carotenoid is the β-carotene which makes up to about 80% of the total carotene. Statistical analysis revealed that no significant difference exists between the mean of each of the processing methods on the carotenoid profile of the oil sample analyzed.
References
[1]
Rees, A.R. (2015) Evidence of the African Origin of Oil Palm Principles. Journal of Life Science, 2, 30-35.
[2]
Bonnie, T.Y. and Choo, Y.M. (2016) Valuables Minor Constituents of Commercial Red Palm Olein; Carotenoid, Vitamin E, Ubiquinones and Sterols. Journal of Oil Palm Research, 12, 14-24.
[3]
Goh, S.H., Choo, Y.M. and Ong, A.S.H. (1985) Minor Constituents of Palm Oil. Journal of the American Oil Chemists’ Society, 62, 237-240. https://doi.org/10.1007/BF02541384
[4]
Edem, D.O. (2002) Palm Oil: Biochemical, Physiological. Nutritional, Hematological, and Toxicological Aspect: A Review. Plant Foods for Human Nutrition, 137, 319-341. https://doi.org/10.1023/A:1021828132707
[5]
Eric, B., Frederic, M., Lucien, H., Elmer, K. and Torsten, B. (2010) Comparison of 3 Spectorphotometric Methods for Carotenoid Determination in Frequently Consumer Fruit and Vegetables. Journal of Food Science, 75, 55-61. https://doi.org/10.1111/j.1750-3841.2009.01417.x
[6]
Faessier, K. (2014) Palm Oil. American Journal of Oil Chemistry Society, 23, 6472-6477.
[7]
Pearson, D.M. (1976) The Chemical Analysis of Foods. 6th Edition, AVI Publishers, West Port.
[8]
AOCS (2005) Official Methods and Recommended Practices of the American Oil Chemists’ Society.
[9]
Yap, S.C., Choo, Y.M., Ooi, C.K., Ong, A.S.H. and Goh, S.H. (1991) Quantitative Analysis of Carotenes in the Oil from Different Palm Species. Journal of Oil Palm Research, 3, 369-378.
[10]
Omokpariola, D.O., Okechukwu, V.U. and Omokpariola, P.L. (2021) Effects of Processing on the Nutritive and Anti-Nutritive Properties of Afzelia africana. Advanced Journal of Chemistry—Section B, 3, 188-198.
[11]
Codex, A. (2011) Codex Standards for Named Vegetable Oils. Codex Publishers, Rome, Vol. 8, 12-22.
[12]
Japir, A.A.W., Salimon, J., Derawi, D., Bahadi, M., Al-Shuja’a, S. and Yusop, M.R. (2017) Physicochemical Characteristics of High Free Fatty Acid Crude Palm Oil. OCL, 24, D506. https://doi.org/10.1051/ocl/2017033
[13]
Okechukwu, V.U., Eze, S.O., Omokpariola, D.O. and Okereke, J.C. (2021) Evaluation of Phytochemical Constituents of Methanol Extract of Moringa oleifera Lam. Whole Leaf by Gas Chromatography-Mass Spectrometry and Fourier Transform Infrared Spectroscopy Analysis. World News of Natural Sciences, 37, 18-30.
[14]
Omokpariola, D.O., Precious-Egere, S.C., Omokpariola, P.L. and Okechukwu, V.U. (2021) Phytochemical and Anti-Microbial Analysis of Metabolites in Seeds of Moringa oleifera Grown in Nigeria. Progress in Chemical and Biochemical Research, 4, 268-277.
[15]
Agatemor, C. (2006) Studies of Selected Physicochemical Properties of Fluted Pumpkin (Telfairia occidentalis Hook F.) Seed Oil and Tropical Almond (Terminalia catappia L.) Seed Oil. Pakistan Journal of Nutrition, 5, 306-307. https://doi.org/10.3923/pjn.2006.306.307
[16]
Suja, K.P., Abraham, J.T., Thamizah, S.N., Jayalekshmy, A. and Arumughan, C. (2004) Antioxidant Efficacy of Sesame Cake Extract in Vegetable Oil Production. Food Chemistry, 84, 393-400. https://doi.org/10.1016/S0308-8146(03)00248-6
[17]
Onwuka, G.I. (2015) Peroxide Value. In: Food Analysis and Instrumentation: Theory and Practical, Naphthali Prints, Lagos, 70-72.
[18]
Aremu, M.O. and Amos, V.A. (2010) Fatty Acids and Physicochemical Properties of Sponge Luffa (Luffa cylindrical) Kernel Oil. International Journal of Chemical Sciences, 3, 161-166.
[19]
Abdulkadir, A.G. and Jimoh, W.L.O. (2013) Comparative Analysis of Physico-Chemical Properties of Extracted and Collected Palm Oil and Tallow. ChemSearch Journal, 4, 44-54.
[20]
Nigerian Institute for Oil and Palm Research (1978-1979) Fifteenth Annual Report. Characteristics of Palm Oil. NIFOR.
[21]
Saini, R.K. and Keum, S.Y. (2018) Significance of Genetic, Environmental, and Pre- and Postharvest Factors Affecting Carotenoid Contents in Crops: A Review. Journal of Agricultural and Food Chemistry, 66, 5310-5324. https://doi.org/10.1021/acs.jafc.8b01613
[22]
Sundram, K., Sambanthamurthi, R. and Tan, Y.A. (2003) Palm Fruit Chemistry and Nutrition. Asia Pacific Journal of Clinical Nutrition, 12, 355-362.
[23]
Roomi, M.W., Niedzwiecki, A. and Rath, M. (2018) Scientific Evaluation of Dietary Factors in Cancer. Journal of Nutritional Medicine and Diet Care, 4, 1-13. https://doi.org/10.23937/2572-3278.1510029
[24]
Zhou, X., Wang, H., Wang, C., Zhao, C., Peng, Q., Zhang, T. and Zhao, C. (2018) Stability and in Vitro Digestibility of Beta-Carotene in Nanoemulsions Fabricated with Different Carrier Oils. Food Science and Nutrition, 6, 2537-2544. https://doi.org/10.1002/fsn3.862
