Pineapple is the first fruit crop cultivated in south Benin that greatly contributes for food and nutritional security and farmers’ income. But the lack of homogenous planting material constitutes the major constraint for improving pineapple yield. In vitro micropropagation is now used in the production of homogenous and free disease planting materials of pineapple. However, the acclimatization to natural condition of pineapple plantlets is an important step in planting material production of this crop. Here, we determined the intrinsic and extrinsic factors which influence the behavior of plantlets during the acclimatization process. For this purpose, plantlets from different categories were selected, trimmed and planted on a horticultural substrate made up of potting soil, white sawdust and compost previously sterilized. The plantlets were then incubated in under acclimatization greenhouse with average temperature of 29˚C and 70.2% of humidity. A batch of plantlets was subjected to two different watering solutions: Shive and Robbins solution and NPK 14-6-5 foliar fertilizer. The results obtained initially showed high rate (100%) of survival and growth of the plantlets watered with Shive and Robbins solution against 50% of the plantlets watered with the foliar fertilizer solution. In addition, the plantlets with spread pores exhibiting the characteristics of which the number of leaves varies between 9 and 11, the weight between 1.2 and 1.5 g, the size of 4.5 to 5.5 cm, and a good junction between the aerial part and the root system were those which were successfully grown in acclimatization phase under greenhouse, unlike plantlets with erected pores having lower success rate. This study goes a long way in providing good procedures of acclimatization of homogenous and free disease planting material of pineapple to the famers.
References
[1]
Loeillet, D. (2005) Cyclope 2005: Les marchés mondiaux. Economica, Paris, 339-341.
[2]
FAOSTAT (Food and Agriculture Organization Corporate Statistical Database) (2021) 2019 Food and Agricultural Organization-statistical Database, Recorded Data for Pineapples Production Quantity in Benin. FAO (Food and Agriculture Organization of the United Nations), Rome. http://www.fao.org/faostat/en/#data/QC
[3]
Mangara, A., N’da Adopo, A.A., Traore, K., Kehe, M., Soro, K. and Toure, M. (2010) Phytoecological Study of Weeds in Pineapple Crops. Journal of Applied Biosciences, 36, 2367-2382.
[4]
Helvetas-Benin (2008) Support to the Organic and Fair Trade Pineapple Sector: Project Document. Helvetas-Benin, Cotonou, 120 p.
[5]
Fassinou Hotegni, V.N., Lommen, W.J.M., van der Vorst, J.G., Agbossou, E.K. and Struik, P.C. (2014) Bottlenecks and Opportunities for Quality Improvement in Fresh Pineapple Supply Chains in Benin. International Food and Agribusiness Management Review, 17, 139-170.
[6]
Agbangba, C.E. (2008) Contribution to the Formulation of Specific Fertilizers for Pineapple Cultivation through Foliar Diagnosis in the Commune of Allada. Thesis of Agricultural Engineer. Faculty of Agronomy, University of Parakou, Benin, 159 p.
[7]
Fassinou Hotegni, V.N., Lommen, W.J.M., van der Vorst, J.G., Agbossou, E.K. and Struik, P.C. (2012) Analysis of Pineapple Production Systems in Benin. Acta Horticulturae, 928, 47-58. https://doi.org/10.17660/ActaHortic.2012.928.4
[8]
Azonkpin, S., Chougourou, C.D. and Soumanou, M.M. (2017) Characteristics of the Technical Itineraries of Pineapple (Ananas comosus var. comosus) Production among Small Producers in the Commune of Allada in Benin. Annales de l’Université de Parakou. Série «Sciences Naturelles et Agronomie», 7, 130-137.
[9]
Tossou, C.C., Capo-Chichi, D.B.E. and Yedomonhan, H. (2015). Diversity and Morphological Characterization of Pineapple Varieties (Ananas comosus (L.) Merrill) Grown in Benin. Journal of Applied Biosciences, 87, 8113-8120. https://doi.org/10.4314/jab.v87i1.2
[10]
Badou, T.B., Pita, S.J., Houedjissin, S.S. and Ahanhanzo, C. (2018) Control of Endogenous Germs by Use of Antimicrobial Agents and Response of Different Explants during the in Vitro Initiation Phase of Pineapple (Ananas comosus (L.) Mill var. Comosus). Journal of Applied Biosciences, 121, 12129-12143. https://doi.org/10.4314/jab.v121i1.4
[11]
Escalona, M., Lorenzo, J.C., González, B., Daquinta, M., Borroto, C., González, J.L. and Desjardines, Y. (1999) Pineapple (Ananas comosus L. Merr) Micropropagation in Temporary Immersion Systems. Plant Cell Reports, 18, 743-748. https://doi.org/10.1007/s002990050653
[12]
Daquinta, M. and Benegas, R. (1997) Brief Review of Tissue Culture of Pineapple. Pineap News, 3, 7-9.
[13]
Botella, J. and Fairbairn, D. (2005) Present and Future Potential of Pineapple Biotechnology. Acta Horticulturae, 622, 23-28. https://doi.org/10.17660/ActaHortic.2005.666.1
[14]
Wang, M.L., Uruu, G., Xiong, L., He, X., Nagai C., Cheah, K., Hu, J., Nan, G.L., Sipes. B. and Atkinson, H. (2009) Production of Transgenic Pineapple (Ananas comosus (L.) Merr.) Plants via Adventitious Bud Regeneration. In Vitro Cellular & Developmental Biology-Plant, 45, 112-121. https://doi.org/10.1007/s11627-009-9208-8
[15]
Loyola-González, O., Medina-Pérez, M.A., Hernández-Ta-mayo, D., Monroy, R., Carrasco-Ochoa, J.A. and García-Borroto, M.A. (2019) Pattern-Based Approach for Detecting Pneumatic Fail-Ures on Temporary Immersion Bioreactors. Sensors, 19, Article No. 414. https://doi.org/10.3390/s19020414
[16]
Parveen, S., Mir, H., Ranjan, T., Pal, A.K. and Kundu, M. (2019) Effect of Surface Sterilants on in Vitro Establishment of Pineapple (Ananas comosus (L.) Merill.) cv. Kew. Current Journal of Applied Science and Technology, 33, 1-6. https://doi.org/10.9734/cjast/2019/v33i230050
[17]
Venancio, J.B., Araújo, W.F. and Chagas, E.A. (2019) Acclimatization of Micropropagated Seedlings of Pineapple Cultivars on Organic Substrates. Científica, 47, 52-61. https://doi.org/10.15361/1984-5529.2019v47n1p52-61
[18]
Yanes-Paz, E., González, J. and Sánchez, R. (2000) A Technology of Acclimatization of Pineapple Vitroplants. Pineap News, 7, 5-6.
[19]
González, R., Laudat, T., Arzola, M., Méndez, R., Marrero, P., Pulido L., Dibut B. and Lorenzo, J.C. (2010) Effect of Azotobacter chroococcum on in Vitro Pineapple Plants’ Growth during Acclimatization. In Vitro Cellular & Developmental Biology-Plant, 47, 387-390. https://doi.org/10.1007/s11627-010-9334-3
[20]
Soto, G., Lorente, G., Mendoza, J., Báez, E.D., Lorenzo, C.M., Rodríguez, R., Hajari, E., Vicente, O., Lorenzo, J.C. and Baez, E. (2020) Growth of Pineapple Plantlets during Acclimatization Can Be Monitored through Automated Image Analysis of the Canopy. The Euro Biotech Journal, 4, 223-229. https://doi.org/10.2478/ebtj-2020-0026
[21]
Mengesha, A., Ayenew, B. and Tadesse, T. (2013) Acclimatization of in Vitro Propagated Pineapple (Ananas comosus (L.), var. Smooth cayenne) Plantlets to ex Vitro Condition in Ethiopia. American Journal of Plant Sciences, 4, 317-323. https://doi.org/10.4236/ajps.2013.42042
[22]
Rodríguez-Escriba, R.C., Rodríguez, R., López, D., Lorente, G.Y., Pino, Y., Aragón, C.E., Garza, Y., Podestá, F.E. and González-Olme-do, J.L. (2015) High Light Intensity Increases the CAM Expression in “MD-2” Micro-Propagated Pineapple Plants at the End of the Acclimatization Stage. American Journal of Plant Sciences, 6, 3109-3118. https://doi.org/10.4236/ajps.2015.619303
[23]
Lorente-González, G.Y., Pino-Legrat, Y., Rodríguez-Escriba, R.C., Pérez-Borroto, L.S., Nápoles-Borrero, L., Mendoza-Ro-dríguez, J., Cardoso, D., Alonso A., Rodríguez Sánchez, R. and González-Olmedo, J. (2018) Foliar Fertilization of “MD-2” Pine Apple Plants (Ananas comosus var. comosus) during the Acclimatization Phase. Newsletter of the Pineapple Working Group, International Society for Horticultural Science, 25, 13-17.
[24]
Badou, B.T., Agbidinoukoun, A., Cacaï, G.T.H., Dossoukpèvi, R.C. and Ahanhanzo, C. (2018) Effects of System Benzylaminopurine-Adenine Sulphate in Combination with Naphthalene Acetic on in Vitro Regeneration and Proliferation of Pineapple (Ananas comosus (L.) Mill var. comosus). American Journal of Biotechnology and Bioscience, 2, 1-15.
[25]
Shive, J.W. and Robbins, W.R. (1942) Sand and Water Culture Methods Used in the Study of Plant Nutrition. In: Hewitt, E.J., Ed., Technical Communication No. 22 of the Commonwealth Bureau of Horticulture and Plantation Crops, Vol. 22, 86.
[26]
Adabe, K.E., Hind, S. and Maïga, A. (2016) Production et Transformation de l’Ananas. Collection Pro-Agro: Wageningen-Pays-Bas, 44 p.
[27]
The French Agricultural Research and International Cooperation Organization for Sustainable Development (CIRAD) (2018) Small Guide for Pineapple Production in French Guiana. Montpellier, 3-31.
[28]
Souza da Silva, L.F. and Reinhardt, D.H. (2006) Pineapple. Embrapa Mandioca e Fruticultura, Cruz das Almas-BA, Brazil, 179-201.
[29]
Padonou, G.E., Aholoukpe, H.N.S., Sossa, E.L., Saidou, A. and Amadji, G.L. (2018) Réponse de l’ananas (Ananas comosus L. Merrill) à la fertilisation minérale élémentaire sur sol ferralitique au Sud du Bénin. International Journal of Biological and Chemical Sciences, 12, 2653-2666. https://doi.org/10.4314/ijbcs.v12i6.15
[30]
Folliot, M. and Marchal, J. (1990) Influence of the Culture Medium on the Growth Rate of Pineapple Plantlets in the Acclimatization Phase. Fruits, 45, 367-378.
[31]
Houssou, V.M.C., Hounsou, B.M., Allé, C.S.Y., Houssou, S., Houssou, S.C. and Agbossou, K.E. (2016) Rainfall Variability and Impact on the Water Balance of Soils under Pineapple Cultivation in South Benin. International Journal of Innovation and Applied Studies, 15, 830-845.
[32]
Konan, D., Bakayoko, A., Piba, S.C., Bitignon, B.G.A. and Trabi, F.H. (2015) Dynamisme de la structure diamétrique du peuplement ligneux des différents biotopes de la forêt classée de Yapo-Abbé, sud de la Côte d’Ivoire. Journal of Applied Biosciences, 94, 8869-8879. https://doi.org/10.4314/jab.v94i1.10
[33]
Fki, L., Kiaa, W., Sahnoun, N., Bouaziz, N., Masmoudi, and Drira, N. (2010) Pilot-Scale Production of Date Palm Vitroplants: Production Schemes and Constraints Treatment. In: Date Palm Biotechnology, IRD Editions, Mar-seille, 195-214.
