Chronic obstructive pulmonary disease (COPD) is a major chronic respiratory disease worldwide. Inflammatory
cells reflect the inflammatory situation both in
peripheral blood and induced sputum. Their correlation has not been reported. The correlation between
neutrophils (Neu), eosinophils (Eos), and lymphocyte (Lym) in induced sputum
and that in peripheral blood of COPD patients
was evaluated to explore
the consistency of inflammatory cells in peripheral blood
and induced sputum. Peripheral blood and induced sputum were collected from 437 patients
with acute exacerbation of COPD (AECOPD) who
were hospitalized in the Department of respiratory and critical care medicine,
Inner Mongolia People’s Hospital. The correlation
analysis was performed by Spearman correlation analysis. The
ratios of Neu,
Eos, and Lym in induced sputum were (79.15 ± 22.60)%, (5.23 ± 12.74)%, and
(1.69 ± 2.66)%. The ratios of Neu, Eos, and Lym in peripheral blood were (63.29 ± 11.44)%, (2.99 ± 3.60)%, and (25.16 ± 10.19)%. The results
showed that the ratios of Neu and Eos in induced sputum were significantly
correlated with the proportion of corresponding cells in peripheral blood (P < 0.05). There was no correlation between the ratio of
Lym and Leu in induced sputum and corresponding cells in peripheral
blood (P > 0.05). In patients with AECOPD, the
tendency of Neu and Eos in induced sputum was consistent with the
corresponding cells in peripheral blood. Neu and Eos in induced
sputum and peripheral blood reflected the degree of inflammation to
guide the individualized medication of patients.
References
[1]
Pleasants, R.A., Heidari, K., Wheaton, A.G., Ohar, J.A., Strange, C., Croft, J.B., et al. (2015) Targeting Persons with or at High Risk for Chronic Obstructive Pulmonary Disease by State-Based Surveillance. COPD, 12, 680-689.
[2]
(2019) Global Strategy for the Diagnosis, Management and Prevention of COPD, Global Initiative for Chronic Obstructive Lung Disease (GOLD).
[3]
Collaborators GBDCRD (2017) Global, Regional, and National Deaths, Prevalence, Disability-Adjusted Life Years, and Years Lived with Disability for Chronic Obstructive Pulmonary Disease and Asthma, 1990-2015: A Systematic Analysis for the Global Burden of Disease Study 2015. The Lancet Respiratory Medicine, 5, 691-706.
https://doi.org/10.1016/S2213-2600(17)30293-X
[4]
(2018) Global Health Estimates 2016: Deaths by Cause, Age, Sex, by Country and by Region, 2000-2016.
[5]
Vogelmeier, C.F., Criner, G.J., Martinez, F.J., Anzueto, A., Barnes, P.J., Bourbeau, J., et al. (2017) Global Strategy for the Diagnosis, Management, and Prevention of Chronic Obstructive Lung Disease 2017 Report. GOLD Executive Summary. American Journal of Respiratory and Critical Care Medicine, 195, 557-582.
https://doi.org/10.1164/rccm.201701-0218PP
[6]
Barnes, P.J. (2016) Inflammatory Mechanisms in Patients with Chronic Obstructive Pulmonary Disease. The Journal of Allergy and Clinical Immunology, 138, 16-27.
https://doi.org/10.1016/j.jaci.2016.05.011
[7]
Strzelak, A., Ratajczak, A., Adamiec, A. and Feleszko, W. (2018) Tobacco Smoke Induces and Alters Immune Responses in the Lung Triggering Inflammation, Allergy, Asthma and Other Lung Diseases: A Mechanistic Review. International Journal of Environmental Research and Public Health, 15, 1033.
https://doi.org/10.3390/ijerph15051033
[8]
Hellermann, G.R., Nagy, S.B., Kong, X., Lockey, R.F. and Mohapatra, S.S. (2002) Mechanism of Cigarette Smoke Condensate-Induced Acute Inflammatory Response in Human Bronchial Epithelial Cells. Respiratory Research, 3, 22.
https://doi.org/10.1186/rr172
[9]
Churg, A., Zay, K., Shay, S., Xie, C., Shapiro, S.D., Hendricks, R., et al. (2002) Acute Cigarette Smoke-Induced Connective Tissue Breakdown Requires Both Neutrophils and Macrophage Metalloelastase in Mice. American Journal of Respiratory Cell and Molecular Biology, 27, 368-374. https://doi.org/10.1165/rcmb.4791
[10]
Wang, Y., Xu, J., Meng, Y., Adcock, I.M. and Yao, X. (2018) Role of Inflammatory Cells in Airway Remodeling in COPD. International Journal of Chronic Obstructive Pulmonary Disease, 13, 3341-3348. https://doi.org/10.2147/COPD.S176122
[11]
Ritchie, A.I. and Wedzicha, J.A. (2020) Definition, Causes, Pathogenesis, and Consequences of Chronic Obstructive Pulmonary Disease Exacerbations. Clinics in Chest Medicine, 41, 421-438. https://doi.org/10.1016/j.ccm.2020.06.007
[12]
Oliver, B., Tonga, K., Darley, D., Rutting, S., Zhang, X., Chen, H., et al. (2019) COPD Treatment Choices Based on Blood Eosinophils: Are We There Yet? Breathe (Sheffield, England), 15, 318-323. https://doi.org/10.1183/20734735.0254-2019
[13]
Tangedal, S., Nielsen, R., Aanerud, M., Persson, L.J., Wiker, H.G., Bakke, P.S., et al. (2019) Sputum Microbiota and Inflammation at Stable State and during Exacerbations in a Cohort of Chronic Obstructive Pulmonary Disease (COPD) Patients. PLoS ONE, 14, e0222449. https://doi.org/10.1371/journal.pone.0222449
[14]
Aaron, S.D., Vandemheen, K.L., Ramsay, T., Zhang, C., Avnur, Z., Nikolcheva, T., et al. (2010) Multi Analyte Profiling and Variability of Inflammatory Markers in Blood and Induced Sputum in Patients with Stable COPD. Respiratory Research, 11, 41. https://doi.org/10.1186/1465-9921-11-41
[15]
Bakakos, P., Schleich, F., Alchanatis, M. and Louis, R. (2011) Induced Sputum in Asthma: From Bench to Bedside. Current Medicinal Chemistry, 18, 1415-1422.
https://doi.org/10.2174/092986711795328337
[16]
Sinden, N.J. and Stockley, R.A. (2013) Proteinase 3 Activity in Sputum from Subjects with Alpha-1-Antitrypsin Deficiency and COPD. European Respiratory Journal, 41, 1042-1050. https://doi.org/10.1183/09031936.00089712
[17]
Jasper, A.E., McIver, W.J., Sapey, E. and Walton, G.M. (2019) Understanding the Role of Neutrophils in Chronic Inflammatory Airway Disease. F1000Research, 8, F1000 Faculty Rev-557. https://doi.org/10.12688/f1000research.18411.1
[18]
Paliogiannis, P., Fois, A.G., Sotgia, S., Mangoni, A.A., Zinellu, E., Pirina, P., et al. (2018) Neutrophil to Lymphocyte Ratio and Clinical Outcomes in COPD: Recent Evidence and Future Perspectives. The European Respiratory Review, 27, Article ID: 170113. https://doi.org/10.1183/16000617.0113-2017
[19]
Gao, J., Chen, B., Wu, S. and Wu, F. (2020) Blood Cell for the Differentiation of Airway Inflammatory Phenotypes in COPD Exacerbations. BMC Pulmonary Medicine, 20, 50. https://doi.org/10.1186/s12890-020-1086-1
[20]
Kinose, D., Ogawa, E., Kudo, M., Marumo, S., Kiyokawa, H., Hoshino, Y., et al. (2016) Association of COPD Exacerbation Frequency with Gene Expression of Pattern Recognition Receptors in Inflammatory Cells in Induced Sputum. The Clinical Respiratory Journal, 10, 11-21. https://doi.org/10.1111/crj.12171
[21]
Hurst, J.R., Vestbo, J., Anzueto, A., Locantore, N., Mullerova, H., Tal-Singer, R., et al. (2010) Susceptibility to Exacerbation in Chronic Obstructive Pulmonary Disease. The New England Journal of Medicine, 363, 1128-1138.
https://doi.org/10.1056/NEJMoa0909883
[22]
Mycroft, K., Krenke, R. and Gorska, K. (2020) Eosinophils in COPD-Current Concepts and Clinical Implications. The Journal of Allergy and Clinical Immunology: In Practice, 8, 2565-2574. https://doi.org/10.1016/j.jaip.2020.03.017
[23]
Proboszcz, M., Mycroft, K., Paplinska-Goryca, M., Gorska, K., Nejman-Gryz, P., Jankowski, P., et al. (2019) Relationship between Blood and Induced Sputum Eosinophils, Bronchial Hyperresponsiveness and Reversibility of Airway Obstruction in Mild-to-Moderate Chronic Obstructive Pulmonary Disease. COPD, 16, 354-361.
https://doi.org/10.1080/15412555.2019.1675150
[24]
Hastie, A.T., Martinez, F.J., Curtis, J.L., Doerschuk, C.M., Hansel, N.N., Christenson, S., et al. (2017) Association of Sputum and Blood Eosinophil Concentrations with Clinical Measures of COPD Severity: An Analysis of the SPIROMICS Cohort. The Lancet Respiratory Medicine, 5, 956-967.
https://doi.org/10.1016/S2213-2600(17)30432-0
[25]
Bafadhel, M., McKenna, S., Terry, S., Mistry, V., Reid, C., Haldar, P., et al. (2011) Acute Exacerbations of Chronic Obstructive Pulmonary Disease: Identification of Biologic Clusters and Their Biomarkers. American Journal of Respiratory and Critical Care Medicine, 184, 662-671. https://doi.org/10.1164/rccm.201104-0597OC
[26]
Papi, A., Bellettato, C.M., Braccioni, F., Romagnoli, M., Casolari, P., Caramori, G., et al. (2006) Infections and Airway Inflammation in Chronic Obstructive Pulmonary Disease Severe Exacerbations. American Journal of Respiratory and Critical Care Medicine, 173, 1114-1121. https://doi.org/10.1164/rccm.200506-859OC
[27]
Postma, D.S. and Rabe, K.F. (2015) The Asthma-COPD Overlap Syndrome. The New England Journal of Medicine, 373, 1241-1249.
https://doi.org/10.1056/NEJMra1411863
[28]
(2021) Global Strategy for the Diagnosis, Management and Prevention of COPD, Global Initiative for Chronic Obstructive Lung Disease (GOLD).
[29]
Bafadhel, M., McKenna, S., Terry, S., Mistry, V., Pancholi, M., Venge, P., et al. (2012) Blood Eosinophils to Direct Corticosteroid Treatment of Exacerbations of Chronic Obstructive Pulmonary Disease: A Randomized Placebo-Controlled Trial. American Journal of Respiratory and Critical Care Medicine, 186, 48-55.
https://doi.org/10.1164/rccm.201108-1553OC
[30]
Scanlon, S.T. and McKenzie, A.N. (2012) Type 2 Innate Lymphoid Cells: New Players in Asthma and Allergy. Current Opinion in Immunology, 24, 707-712.
https://doi.org/10.1016/j.coi.2012.08.009
[31]
De Grove, K.C., Provoost, S., Verhamme, F.M., Bracke, K.R., Joos, G.F., Maes, T., et al. (2016) Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung. PLoS ONE, 11, e0145961.
https://doi.org/10.1371/journal.pone.0145961
[32]
Bateman, E.D., Reddel, H.K., van Zyl-Smit, R.N. and Agusti, A. (2015) The Asthma-COPD Overlap Syndrome: Towards a Revised Taxonomy of Chronic Airways Diseases? The Lancet Respiratory Medicine, 3, 719-728.
https://doi.org/10.1016/S2213-2600(15)00254-4
[33]
Hogg, J.C., Chu, F., Utokaparch, S., Woods, R., Elliott, W.M., Buzatu, L., et al. (2004) The Nature of Small-Airway Obstruction in Chronic Obstructive Pulmonary Disease. The New England Journal of Medicine, 350, 2645-2653.
https://doi.org/10.1056/NEJMoa032158
[34]
Costa, C., Rufino, R., Traves, S.L., Lapa, E.S.J.R., Barnes, P.J. and Donnelly, L.E. (2008) CXCR3 and CCR5 Chemokines in Induced Sputum from Patients with COPD. Chest, 133, 26-33. https://doi.org/10.1378/chest.07-0393
[35]
Barnes, P.J. (2017) Cellular and Molecular Mechanisms of Asthma and COPD. Clinical Science (London, England: 1979), 131, 1541-1558.
https://doi.org/10.1042/CS20160487
[36]
Tzanakis, N., Chrysofakis, G., Tsoumakidou, M., Kyriakou, D., Tsiligianni, J., Bouros, D., et al. (2004) Induced Sputum CD8+ T-Lymphocyte Subpopulations in Chronic Obstructive Pulmonary Disease. Respiratory Medicine, 98, 57-65.
