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Au@WP5光电化学传感器的制备及其对牛血红蛋白的分析应用
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Abstract:
目的：本研究将水溶性柱[5]芳烃(WP5)修饰在金纳米粒子(Au nanoparticles, AuNPs)表面，作为复合材料修饰玻碳电极(GCE)，建立了一种检测牛血红蛋白的光电化学传感方法。利用Au NPs在可见光照下的局域表面等离子共振效应，WP5与多巴胺(DA)的主客体络合作用，及这二者的协同作用，检测溶液中的多巴胺。由于多巴胺与牛血红蛋白(BHb)的吸附作用，向DA中依次滴加不同浓度的牛血红蛋白。随着牛血红蛋白的加入，多巴胺产生的电信号会减弱，从而达到对牛血红蛋白的间接性检测。结果表明，在牛血红蛋白的浓度为10？11~10？4 mg/mL浓度范围内，阳极峰电流密度与牛血红蛋白的浓度呈较好的线性关系，检出限为5.2 × 10？12 mg/mL (S/N = 3)。
Purpose: In this study, a water-soluble pillar[5] arenes (WP5) were modified on the surface of gold nanoparticles (Au nanoparticles, Au NPs) as a composite material modified glassy carbon electrode (GCE), and their photoelectrochemical sensor was established for detecting bovine hemoglobin. The enhanced photocurrent signal benefited from the localized surface plasmon resonanc (LSPR) effect of Au, the host-guest complexation between WP5 and DA. With the addition of bovine hemoglobin, the electrical signal produced by dopamine will be weakened, so as to achieve indirect detection of bovine hemoglobin. The PEC immunosensor showed a specifically recognize toward BHb with a wide detection range of 1.0 × 10？11 to 10？4 mg/mL and a detection limit of 5.2 × 10？12 mg/mL (S/N = 3).
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