低温冷冻保存对猪精子的影响
Effect of Cryopreservation on Pig Sperm

DOI: 10.12677/BP.2021.113005, PP. 38-45

Keywords: 猪精子，低温冷冻保存
Pig Sperm, Cryopreservation

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Abstract:

精子长期保存的最佳方法是低温冷冻保存，该方法具有保存时间长、生产成本低等优点。在养猪生产中广泛使用此项技术对精子进行长期保存，但由于猪精子质膜上的胆固醇/磷脂较低，所以较其他物种更容易受到低温的损伤，从而导致冷冻后的猪精子活力降低、受精率降低。本文就低温冷冻保存对猪精子超微结构、能量代谢等方面的影响进行了综述，阐明冻融精子的损伤机理，为提高冻精的活力奠定基础。
The best method for long-term storage of semen is cryopreservation, which has the advantages of long storage time and low production cost. This technology is widely used in pig production for long-term preservation of sperm. But due to the low cholesterol/phospholipid on the plasma membrane of pig sperm, therefore, compared with other species, they are more vulnerable to low temperature damage, resulting in decreased sperm motility and fertilization rate. In this paper, the effects of cryopreservation on the ultrastructure and energy metabolism of porcine sperm were reviewed, and the damage mechanism of frozen thawed sperm was elucidated, so as to lay a foundation for improving the vitality of frozen sperm.

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