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Botanical Research 2021
露白引发对黑籽南瓜种子萌发整齐度的影响研究
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Abstract:
嫁接是瓜类蔬菜克服土传病害、提高抗逆性的有效方法,生产上广泛采用嫁接的方法进行黄瓜生产,黑籽南瓜种子是广泛应用的瓜类砧木种子。嫁接要求砧木幼苗整齐一致,本研究通过引发黑子南瓜种子至刚露白再回干,并通过露白时间早晚调整播期来提高幼苗的整齐度。研究发现,引发超过最佳时间到种子裂口或露白并未显著影响种子再次萌发和幼苗生长,露白引发后可以剔除不发芽种子,确保播种幼苗发芽率达98%以上;通过晚露白的提前播种,早露白的延后播种的分批播种方式,与正常播种相比,可以使发芽结束时黑籽南瓜幼苗的苗高和茎粗更均匀,更符合嫁接要求。通过引发种子的电导率检测发现,露白引发并未显著影响种子的电导率。综上,露白引发结合分批播种可以显著提高幼苗的整齐度,对种子的成苗率也可以有较为准确的估算,是解决发芽不整齐的有效方法。
Grafting is an effective method for melon vegetables to overcome soil-borne diseases and im-prove their resistance to stress. Grafting is widely used in the production of cucumbers, and the Cucurbita ficifolia is widely used in cucumber rootstock. Grafting requires size uniformity of rootstock seedlings matching scions. In this study, the uniformity of seedlings was improved by priming the seeds of Cucurbita ficifolia when they were just exposed radicle, and by adjusting the sowing date by seed breaking testa time sooner or later. The results show that the seed ger-mination and seedling growth were not significantly affected by the seed priming to seed dehis-cence or breaking through testa. The seed germination rate could be more than 98% after breaking through testa priming. Compared with normal sowing, the seedling height and stem diameter of Cucurbita ficifolia can be more uniform at the end of germination and more in line with grafting requirements through the method of sowing in advance for germinate late seeds and sowing delay for early germinate seeds, compared with the method of sowing at the same time. It was found that the electrical conductivity of the seeds was not significantly affected by breaking through testa priming. In conclusion, the combination of breaking through test priming and sowing seperately can significantly improve the uniformity of seedlings and provide a more accurate estimation of the seedling formation rate of seeds, which is an effective method to solve the irregular germination.
[1] | 周士刚. 蔬菜嫁接育苗需注意哪些问题[N]. 河北科技报, 2011-10-25(B05). |
[2] | 刘爱群, 李春艳, 赵丽丽, 王国政, 赵越. 黄瓜插接法嫁接育苗易出现的问题及相应技术调控[J]. 园艺与种苗, 2013(4): 9-11. |
[3] | 马瑞霞, 王彦荣. 种子水引发的研究进展[J]. 草业学报, 2008, 17(6): 143-149. |
[4] | Becerra-Vázquez, á.G., Coates, R., Sán-chez-Nieto, S., Reyes-Chilpa, R. and Orozco-Segovia, A. (2020) Effects of Seed Priming on Germination and Seedling Growth of Desiccation-Sensitive Seeds from Mexican Tropical Rainforest. Journal of Plant Research, 133, 855-872. (Prepublish) https://doi.org/10.1007/s10265-020-01220-0 |
[5] | 姚东伟, 吴凌云, 沈海斌, 田守波, 李明. 种子引发技术研究与应用进展[J]. 上海农业学报, 2020, 36(5): 153-160. |
[6] | 罗先洋. 水引发对猕猴桃种子萌发和幼苗生长的影响[D]: [硕士学位论文]. 合肥: 安徽农业大学, 2020. |
[7] | Miladinov, Z., Maksimovic, I., Balesevic Tubic, S., Djukic, V., Canak, P., Miladinovic, J. and Djordjevic, V. (2020) Priming Seed Mitigates the Effects of Saline Stress in Soybean Seedlings. Legume Research, 43, 263-267.
https://doi.org/10.18805/LR-469 |
[8] | 查昱, 张宗豪, 徐海峰, 刘欣, 李玉玲, 李秀璋. 水引发对垂穗披碱草种子萌发及幼苗生长的影响[J/OL]. 东北农业科学: 1-7. http://kns.cnki.net/kcms/detail/22.1376.S.20210507.1713.012.html, 2021-05-15. |
[9] | 张琛. 种子引发与丸粒化对油菜萌发及生长发育的影响[D]: [硕士学位论文]. 武汉: 华中农业大学, 2019. |
[10] | Malek, M., Ghaderi-Far, F., Torabi, B., Sadeghipour, H.R. and Hay, F.R. (2019) The Influence of Seed Priming on Storability of Rapeseed (Brassica napus) Seeds. International Seed Testing Association, 47, 87-92.
https://doi.org/10.15258/sst.2019.47.1.09 |
[11] | 向莹莹, 李浩卓, 张婷婷, 王建华, 孙群. 电导率法早期检测玉米和小麦种子活力[J]. 中国农业大学学报, 2020, 25(6): 12-19. |
[12] | 刘春香, 刘军禄, 黄明杰, 孙建福, 刘长林. 萌发后回干对种子耐贮藏性和再萌发的影响研究[J]. 植物学研究, 2018, 7(3): 294-304. |
[13] | 寇雷. 适用不同直径配比的自动化硬枝嫁接装置设计[D]: [硕士学位论文]. 保定: 河北农业大学, 2021. |
[14] | 王文恩, 孟盛旺, 张莎, 张冬丽, 陈功. 水引发和非生物胁迫对多花木蓝种子萌发的影响[J]. 安徽农业科学, 2016, 44(5): 180-184. |
[15] | 李志宇, 杨明挚, 杨春燕, 梅柱华. 不同引发回干处理对滇龙胆种子萌发的影响[J]. 时珍国医国药, 2014, 25(6): 1480-1484. |
[16] | 王爱民, 邹瑞昌, 鞠丽萍, 杜纪艳. 常见蔬菜嫁接育苗及栽培关键技术[J]. 长江蔬菜, 2020(23): 37-40. |
[17] | 刘忠奇, 贺记外, 张海清, 刘爱民. 植物种子脱水耐性的研究现状分析与展望[J]. 中国农学通报, 2020, 36(2): 36-41. |
[18] | 胡晋. 种子检验学[M]. 北京: 科学出版社, 2018: 188-190, 223. |
[19] | Khan, M.N., Khan, Z., Luo, T., Liu, J., Rizwan, M., Zhang, J., Xu, Z., Wu, H. and Hu, L. (2020) Seed Priming with Gibberellic Acid and Melatonin in Rapeseed: Consequences for Improving Yield and Seed Quality under Drought and Non-Stress Conditions. Industrial Crops & Products, 156, Article ID: 112850.
https://doi.org/10.1016/j.indcrop.2020.112850 |