条斑紫菜氨基酸组成变化及与栽培海区氮含量相关性研究
The Study on Variation of Amino Acids in Phycocalidia yezoensis and the Correlation Analysis with the Nitrogen Content in Culture Area

DOI: 10.12677/HJAS.2021.117091, PP. 678-689

Keywords: 条斑紫菜，氨基酸，差异性，氮盐
Phycocalidia yezoensis, Amino Acids, Variation, Nitrogen

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Abstract:

对2018~2019年度江苏沿海海域5个站位、5个采收期(剪收次数)的条斑紫菜中氨基酸含量及对应的海水氮含量进行了检测，并分析了条斑紫菜中氨基酸的变化规律及其与海水氮含量的相关性。结果表明，5个站位条斑紫菜中的18种氨基酸具有不同程度的差异性，在各个站位间均呈极显著差异的氨基酸有天冬氨酸、丙氨酸、缬氨酸及脯氨酸；5个采收期条斑紫菜中的氨基酸含量总体上是差异显著的(P < 0.05)；其必需氨基酸之和在不同站位间差异不显著(P > 0.05)，在不同采收期间差异极显著(P < 0.01)；总氨基酸含量除12月与2月份之间的无显著差别外，其它采收期之间均有极显著区别(P < 0.01)，12月与4月、1月与3月、1月与4月、2月与4月的条斑紫菜样品中18种氨基酸均呈极显著差异(P < 0.01)。海水中氮盐与条斑紫菜中氨基酸显著相关性的列为总无机氮 > 硝态氮 > 氨态氮。
This study investigated the variation of amino acids in Phycocalidia yezoensis at 5 stations, 5 har-vest periods from 2018 to 2019 in Jiangsu Province, and analyzed the correlation between the ami-no acids of samples and the nitrogen content in the water. The results showed that 18 kinds of amino acids of samples were various between 5 stations, among them, aspartic acid, alanine, valine and proline were presented highly significant difference in each station. The total amino acids showed highly significant difference between each other harvest period except the group between the December and the February, in addition, 18 amino acid presented highly significant difference in the groups between December and April, January and March, January and April, February and April. The content of essential amino acid in Phycocalidia yezoensis presented highly significant difference between the harvest period, and no significant difference between the stations. The ranking of the relevance was TN > NO₃_N > NH₄+-N.

References

[1]	马家海, 蔡守清. 条斑紫菜的栽培与加工[M]. 北京: 科学出版社, 1996: 132-137.
[2]	Noda, H., et al. (1975) Studies on the Flavor Substances of Nori Prophyra tenera. Bulletin of Japanese of Scientific Fisheries, 41, 1299-1303.
[3]	王海明, 周彦钢, 任玉翠. 条斑紫菜营养成分分析[J]. 浙江省医学科学院学报, 1997, 8(2): 24-25.
[4]	陈必链, 林跃鑫, 黄键. 坛紫菜的营养评价[J]. 中国海洋药物. 2001, 20(2): 51-53.
[5]	张全斌, 赵婷婷, 綦慧敏, 李智恩, 徐祖洪. 紫菜的营养价值研究概况[J]. 科学视野, 2005, 29(2): 69-72.
[6]	马家海, 大住幸宽, 川合正允. 浙江省象山港紫菜轮栽及其品质分析的研究[J]. 中国水产科学, 1997, 4(1): 31-33,35-38.
[7]	何培民, 王素娟. 条斑紫菜细胞苗培养及总氮量、氨基酸分析[J]. 水产学报, 1991, 15(1): 48-54.
[8]	Takagi, M. and Kuriyama, M. (1959) Chemical Studies on Marine Algae. Ⅻ. The Free Amino Acids in Several Species of Marine Algae. Bulletin of the Faculty of Fisheries Hokkaido University, 10, 72-76
[9]	Yoshie, Y., Suzuki, T. and Shirai, T. (1993) Free Amino Acids and Fatty Acid Composition in Dries Nori of Various Culture Locations and Prices. Nippon suisan gakkaishi, 59, 1769-1775.
[10]	李瑞霞, 伊纪峰, 沈颂东, 刘海勇, 龚兴权, 郑佳亮, 等. 条斑紫菜(Porphyra ye-zoensis)游离氨基酸组成分析[J]. 氨基酸和生物资源, 2011, 33(1): 4-9.
[11]	Fleurence, J. (1999) Seaweed Proteins: Biochemical, Nutritional Aspects and Potential Uses. Trends in Food Science & Technology, 10, 25-28. https://doi.org/10.1016/S0924-2244(99)00015-1
[12]	马飞, 陆勤勤, 胡传明, 张涛, 周伟, 李家富. 多个条斑紫菜品系采收期内脂肪酸组成、含量的变化分析[J]. 水产学报, 2013, 37(10): 1551-1557.
[13]	Sakai, H. and Kasai, T. (2000) Fatty Acids, Free Amino Acids and 5’-Nucleotides of Dried Laver, Hoshi-Nori, Harvested in Different Months in Hokkaido and Produced under Different Drying Conditions. Nippon Shokuhin Kagaku Kogaku Kaishi, 47, 327-332.
[14]	胡传明, 陆勤勤, 张美如, 朱建一, 马飞, 周伟, 等. 不同品系条斑紫菜采收期游离氨基酸组成与含量变化特征[J]. 江苏农业科学, 2015, 43(7): 334-337.
[15]	纪明侯, 蒲淑珠, 牛伫庆. 不同海区生长的条斑紫菜的氨基酸含量变化[J]. 海洋与湖沼, 1981, 12(6): 522-530.
[16]	曾繁杰, 蒋丽金, 徐秀璋, 张伟, 林裕水. 中国坛紫菜和条斑紫菜中的氨基酸组成和营养价值的研究[J]. 海洋与湖沼, 1991, 22(6): 590-593
[17]	Lapointe, B.E. (1987) Phosphorus and Nitrogen Limited Photosynthesis and Growth of Gracilaria tikvahiae (Phodophyceae) in the Florida Keys: An Experimental Field Study. Marine Biology, 93, 561-568. https://doi.org/10.1007/BF00392794
[18]	Niwa, K., Furuita, H. and Yamamoto, T. (2008) Changes of Growth Characteristics and Free Amino Acid Content of Cultivated Pyropia yezoensis Ueda (Bangiales Rhodophyta) Blades with the Progression of the Number of Harvests in a Nori Farm. Journal of Applied Phycology, 20, 687-693. https://doi.org/10.1007/s10811-007-9273-5
[19]	Chiang, Y.M. and Lin, S.H. (1988) Studies on the Effects of Ni-trogen and Salinity on the Growth of the Conchocelis of Pyropia haitanensis Chang and Zheng in Culture. Journal of the Fisheries Society of Taiwan, 16, 189-195.
[20]	Iwasaki, H. (1967) Nutritional Studies of the Edible Seaweed Pyropia tenera. Ⅱ. Nutrition of Conchocelis. Journal of Phycology, 3, 30-34. https://doi.org/10.1111/j.1529-8817.1967.tb04625.x
[21]	Chapman, A.R. and Craigie, J.S. (1977) Seasonal Growth in Laminaria longicuris Relations with Dissolved Inorganic Nutrients and Internal Reserves of Nitrogen. Marine Biology, 40, 197-205. https://doi.org/10.1007/BF00390875

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