叶面喷施壳寡糖对烤烟质体色素及其降解物质的影响
Effects of Chitooligosaccharide on Plastid Pigment and Its Degradation Products in Flue-Cured Tobacco Leaves

【目的】研究烤烟叶面喷施壳寡糖对烤烟质体色素及其降解物质的影响，旨在提高广元烤烟香气品质，探索新的农艺化控手段。【方法】以云烟87为供试品种，采取随机区组试验，设置5个处理，每个处理三次重复，在喷施过程中，各个处理喷施壳寡糖的量相同浓度不同。【结果】随着喷施壳寡糖浓度的增加，烤烟上中部叶叶绿素的含量呈先降低后升高再降低的趋势，T2 (喷施150 mg/L)处理时显著降低，且最符合优质烟叶对叶绿素含量的要求。上中部烟叶类胡萝卜素含量在T1 (喷施100 mg/L)和T2 (喷施150 mg/L)处理时均有所升高，上中部叶质体色素降解物质总量均在T2 (喷施150 mg/L)和T3 (喷施200 mg/L)处理时明显增多，分别为873.73 μg/g、861.45 μg/g、889.79 μg/g和872.43 μg/g。【结论】烤烟喷施150 mg/L (T2处理)壳寡糖时，可以显著降低烤烟叶绿素含量，提高类胡萝卜素含量和质体色素降解物质总量。
[Objective] To study the effect of chitooligosaccharide on the plastid pigment and its degradation products of flue-cured tobacco leaves, in order to improve the quality and economic benefits of Guangyuan flue-cured tobacco , and to explore new agronomic control methods. [Methods] Yunyan 87 was used as the test variety, and the randomized block experiment was carried out. Five treatments were set up, each treatment was repeated three times. During the spraying process, the same concentration of chitosan oligosaccharide was different for each treatment. [Results] With the increase of chitosan concentration, the content of chlorophyll in the upper and middle leaves of flue-cured tobacco decreased first, then increased, and then decreased. T2 (spraying 150 mg/L) treatment significantly reduced the content of chlorophyll, which was the most in line with the requirements of high-quality tobacco leaves. The content of carotenoid in the upper and middle leaves increased with the increase of spraying concentration. The degradation products of plastid pigment in the upper and middle leaves of flue-cured tobacco were all in T2 (spraying 150 mg/L) and T3 (spraying 200 mg/L) treatment. [Conclusion] The content of chlorophyll, the content of carotenoids and the total amount of degradation products of plastid pigment in flue-cured tobacco can be significantly reduced by spraying 150 mg/L (T2) chitosan oligosaccharide.