基于网络药理学和分子对接探讨槐花抗2型糖尿病作用机制研究
Study on Anti-Type 2 Diabetes Mechanism of Sophora japonica L. based on Network Pharmacology and Molecular Docking

DOI: 10.12677/PI.2021.104025, PP. 193-202

Keywords: 槐花，2型糖尿病，作用机制，网络药理学，分子对接
Sophora Japonica L., Type 2 Diabetes, Mechanism of Action, Network Pharmacology, Molecular Docking

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Abstract:

目的：槐花具有抗2型糖尿病的潜力，但其作用机制尚不明确。方法：通过TCMSP检索筛选槐花的活性成分及靶点，在GeneCards数据库中寻找2型糖尿病的相关靶点，取交集基因进行蛋白互作分析发现，GO生物富集及Kegg富集分析，最后采用分子对接进行验证。结果：得到槐花的活性成分有6个，取交集基因得到93个，蛋白互作分析发现JUN、TP53和MAPK1治疗2型糖尿病的作用，Kegg富集结果发现，槐花治疗2型糖尿病主要通过pathways in cancer、HIF-1 signaling pathway等信号通路进行调控。分子对接结果表明，槐花中的活性成分kaempferol、beta-sitosterol、quercetin等成分与JUN、TP53和MAPK1具有较好的结合能力。结论：槐花可能是通过kaempferol、beta-sitosterol、quercetin等化学成分调控HIF-1 signaling pathway等信号通路上的JUN、TP53和MAPK1等基因发挥治疗2型糖尿病作用。
Objective: Sophora japonica L. has the potential of antitype 2 diabetes, but its mechanism is not clear. Methods: Tcmsp was used to search and screen the active components and targets of Sophora japonica L., and the related targets of type 2 diabetes mellitus were found in GeneCards database. Protein interaction analysis was carried out on the cross genes. Go (BP) and KEGG enrichment analyses were carried out. Finally, molecular docking was used to verify the results. Results: There were 6 active components of Sophora japonica L., and 93 of them were obtained by taking the intersection genes. Protein interaction analysis revealed the effect of JUN, TP53 and MAPK1 in the treatment of type 2 diabetes. KEGG enrichment results showed that the treatment of type 2 diabetes by Sophora japonica L. was mainly regulated by Pathways in cancer and HIF-1 signaling pathway. Molecular docking results showed that active components such as kaempferol, beta-sitosterol and quercetin of Sophora japonica L. had the good binding ability with JUN, TP53 and MAPK1. Conclusion: A. sophorae L .may play an important role in the treatment of type 2 diabetes mellitus by regulating the genes of JUN, TP53 and MAPK1 in HIF-1 signaling pathway and other chemical components such as kaempferol, beta-sitosterol and quercetin.

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