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滇池土著沉水植物根际可培养解磷菌与环境因子相关性研究
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Abstract:
为探究云南滇池4种土著沉水植物根际沉积物中可培养解磷菌群落结构和环境因子的相互关系。采用功能筛选培养的方法对15个沉水植物的根际和非根际沉积物样品中进行解磷菌分离培养，应用16S rDNA序列对分离得到的解磷菌进行系统发育分析，运用软件Biodap和冗余分析(redundancy analysis, RDA)分析解磷菌群落的丰富度及其与环境因子间的相关性。结果显示，从滇池沉水植物根际和非根际沉积物中共分离到可培养解磷菌86株，从属于8个属27个种；其中假单胞菌属(Pseudomonas)为优势菌群，占分离的22%，分布于该属的11个种；杆菌属(Bacillus)占分离总数的18%，共分离到3个种。滇池不同沉水植物根际沉积物中解磷菌的优势类群存在一定差异。冗余分析(RDA)的结果表明：沉水植物根际的沉积物中氧化还原电位(Eh)和沉积物间隙水的溶解性活性磷(SRP)对解磷菌群落结构有显著影响。
In order to explore the community structure and environmental factor characteristics of culturable phosphate-solubilizing bacteria (PSB) in the rhizosphere sediments of four native submerged plants in Dianchi Lake, Yunnan province. [Method] By using the method of functional screening medium and basing on the 16S rDNA sequence phylogenetic analysis of PSB, the PSB strains were isolated and identified from the rhizosphere and non-rhizosphere sediments and using the software BIODAP and Canoco analysis the diversity of PSB and the relationship between the environmental factors. The results showed that 86 strains of PSB were cultured and isolated, belonging to 27 species and 8 genera. Among them, the Pseudomonas was the dominant bacterium, accounting for 22% and distributed in 11 species of this genus. The Bacillus accounted for 18% of the total isolated species, and 3 species were isolated. The community structure of PSB was significantly different in the rhizosphere and non-rhizosphere sediments of the submerged plants in Dianchi Lake. RDA analysis found that the REDOX potential (Eh) and the soluble active phosphorus (SRP) were the main environmental factors for the distribution of PSB.
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