In this study, we aimed to use a novel approach to overcome the current limitations of ozone therapy in medicine through ozonized oil nanoemulsions (OZNEs). We evaluated dose-dependency on the cellular activities of B-16 melanoma cell line which were incubated with various OZNE doses (v/v). Antitumor effects of OZNE against cancer cell lines were evaluated by cellular morphology, apoptosis and cell cycle analysis. Flow cytometry results showed that OZNE induced DNA damage, apoptosis, and arrested cell cycle in G0-1 phase in B-16 melanoma cells. Thus, OZNE treatment could pose an effective way to act as a potential therapeutic for patients with tumors in the future.
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