White spot syndrome virus (WSSV) is one of the most important pathogens in the world. Since its outbreak in 1993, the virus has caused huge economic losses. Studies have confirmed that in the early stage of infection, VP28, the main envelope protein of WSSV, as a viral adhesion protein, binds to PcRab7 of Penaeus chinensis to help the virus enter the host cells. Understanding the mechanism of PcRab7-VP28 interaction is of great significance to understand the mechanism of WSSV infection and the development of antiviral drugs. In this research, the interaction interface and interaction sites were predicted by using the methods of molecular simulations. Results showed that VP28 binds to the second β-sheet (L73-D86) of PcRab7, which is consistent with the region detected in previous studies. Furthermore, we speculated the possible interaction sites in PcRab7 are E81, F77 and D76. These results may contribute to a deep understanding of the infection mechanism of WSSV on the host.
References
[1]
OIE (2003) Manual of Diagnostic Tests for Aquatic Animals, White Spot Disease. Office International des Epizooties, Paris, Chapter 4.1.2.
[2]
Thitamadee, S., Prachumwat, A., Srisala, J., et al. (2016) Review of Current Disease Threats for Cultivated Penaeid Shrimp in Asia. Aquaculture, 452, 69-87.
https://doi.org/10.1016/j.aquaculture.2015.10.028
[3]
Rodriguez-Anaya, L.Z., Gonzalez-Galaviz, J.R., Casillas-Hernandez, R., et al. (2016) Draft Genome Sequence of White Spot Syndrome Virus Isolated from Cultured Litopenaeus vannamei in Mexico. Genome Announcement, 4, e01674-15.
https://doi.org/10.1128/genomeA.01674-15
[4]
Xie, X. and Yang, F. (2006) White Spot Syndrome Virus VP24 Interacts with VP28 and Is Involved in Virus Infection. Journal of General Virology, 87, 1903.
https://doi.org/10.1099/vir.0.81570-0
[5]
Sun, L., Su, Y., Zhao, Y., et al. (2016) Crystal Structure of Major Envelope Protein VP24 from White Spot Syndrome Virus. Scientific Reports, 6, Article No. 32309.
https://doi.org/10.1038/srep32309
[6]
Sritunyalucksana, K., Wannapapho, W., Lo, C.F. and Flegel, T.W. (2006) PmRab7 Is a VP28-Binding Protein Involved in White Spot Syndrome Virus Infection in Shrimp. Journal of Virology, 80, 10734-10742. https://doi.org/10.1128/JVI.00349-06
[7]
Tang, X., Zhai, F., Sheng, X., et al. (2017) The Roles of β-Integrin of Chinese Shrimp (Fenneropenaeus chinensis) in WSSV Infection. International Journal of Molecular Sciences, 18, 1465. https://doi.org/10.3390/ijms18071465
[8]
Krishnankutty Chandrika, S. and Thavarool Puthiyedathu, S. (2021) Challenges and Prospects of Viral Envelope Protein VP28-Based Control Strategies to Combat White Spot Syndrome Virus in Penaeid Shrimps: A Review. Reviews in Aquaculture, 13, 734-743. https://doi.org/10.1111/raq.12497
[9]
Momtaz, F., Foysal, J., Rahman, M. and Fotedar, R. (2019) Design of Epitope Based Vaccine against Shrimp White Spot Syndrome Virus (WSSV) by Targeting the Envelope Proteins: An Immunoinformatic Approach. Turkish Journal of Fisheries and Aquatic Sciences, 19, 149-159. https://doi.org/10.4194/1303-2712-v19_2_07
[10]
van Hulten, M.C.W., Goldbach, R.W. and Vlak, J.M. (2000) Three Functionally Diverged Major Structural Proteins of White Spot Syndrome Virus Evolved by Gene Duplication. Journal of General Virology, 81, 2525-2529.
https://doi.org/10.1099/0022-1317-81-10-2525
[11]
Zhang, X., Huang, G., Xu, X. and Hew, C.L. (2002) Identification and Localization of a Prawn White Spot Syndrome Virus Gene That Encodes an Envelope Protein. Journal of General Virology, 83, 1069. https://doi.org/10.1099/0022-1317-83-5-1069
[12]
Yi, G., Wang, Z., Qi, Y., et al. (2004) Vp28 of Shrimp White Spot Syndrome Virus Is Involved in the Attachment and Penetration into Shrimp Cells. Journal of Biochemistry & Molecular Biology, 37, 726-734.
https://doi.org/10.5483/BMBRep.2004.37.6.726
[13]
Verma, A.K., Gupta, S., Singh, S.P. and Nagpure, N.S. (2017) An Update on Mechanism of Entry of White Spot Syndrome Virus into Shrimps. Fish & Shellfish Immunology, 67, 141-146. https://doi.org/10.1016/j.fsi.2017.06.007
[14]
van Hulten, M.C.W., Witteveldt, J., Snippe, M. and Vlak, J.M. (2001) White Spot Syndrome Virus Envelope Protein VP28 Is Involved in the Systemic Infection of Shrimp. Virology, 285, 228-233. https://doi.org/10.1006/viro.2001.0928
[15]
Gouet, P., Robert, X. and Courcelle, E. (2003) ESPript/ENDscript: Extracting and Rendering Sequence and 3D Information from Atomic Structures of Proteins. Nucleic Acids Research, 31, 3320-3323. https://doi.org/10.1093/nar/gkg556
[16]
Yang, J., Yan, R., Roy, A., et al. (2014) The I-TASSER Suite: Protein Structure and Function Prediction. Nature Methods, 12, 7-8. https://doi.org/10.1038/nmeth.3213
[17]
Laskowski, R.A., MacArthur, M.W., Moss, D.S. and Thornton, J.M. (1993) PROCHECK: A Program to Check the Stereochemical Quality of Protein Structures. Journal of Applied Crystallography, 26, 283-291.
https://doi.org/10.1107/S0021889892009944
[18]
Pierce, B.G., Hourai, Y. and Weng, Z. (2011) Accelerating Protein Docking in ZDOCK Using an Advanced 3D Convolution Library. PLoS ONE, 6, e24657.
https://doi.org/10.1371/journal.pone.0024657
