Enteroviruses are
responsible for emerging diseases which cause diverse symptoms and may result
in neurological complications. An antiviral with multiple mechanisms of action
can help prevent enterovirus mediated disease despite differences in the
pathogenesis between enteroviruses, including the recently identified
enterovirus 69 (EV-69) for which pathogenesis is not well understood. This
study investigated the efficacy of epigallocatechin-3-gallate stearate (EGCG-S),
a modified form of the antioxidant green tea catechin
epigallocatechin-3-gallate (EGCG), in inhibiting EV-69 infection of lung
fibroblast cells in vitro. Treatment
with EGCG-S resulted in moderate protection from EV-69 mediated cytotoxicity as
demonstrated by increased metabolic activity as well as maintenance of cell
morphology and mitochondrial function. These effects were correlated with
reduced hydrogen peroxide production in infected cells following EGCG-S
treatment with concentrations less than 100 μM, suggesting a role for
inhibition of EV-69 mediated oxidative stress. This study provides insight into
characteristics of EV-69 infection as well as the efficacy of EGCG-S mediated
inhibition of EV-69 infection.
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