菌株Marasmius tricolor 310b发酵粗酶液对3种染料脱色效果的研究 Study on Decolorization Effect of Three Dyes by Fermentation Crude Enzyme Solution of Marasmius tricolor 310b
为探索分离自青藏高原高寒草地产漆酶新真菌菌株Marasmius tricolor 310b对苯甲烷类、蒽醌类和偶氮类染料的脱色效果，采用摇瓶发酵法获得漆酶粗酶液进行脱色实验。结果表明菌株Marasmius tricolor 310b漆酶发酵粗酶液都能够有效的降解溴甲酚绿(苯甲烷类)、茜素红(蒽醌类)、刚果红(偶氮类) 3种染料，但降解效果有所差异，其中，以苯甲烷类染料脱色作用最优。另发现菌株Marasmius tricolor 310b发酵粗酶液对3种染料的降解程度与波长和反应时间有直接关系，随着反应时间的延长，降解效率有不同程度提高。我们的研究初步揭示了新菌株Marasmius tricolor 310b发酵粗酶液对苯甲烷类、蒽醌类、偶氮类具有明显的脱色效果，这一发现对于更新具有脱色效果的真菌资源库有一定的理论支持。
In order to explore the decolorization effect of a new laccase producing fungus, Marasmius tricolor 310b, isolated from the alpine grassland of Qinghai-Tibet Plateau on phenylmethane, anthraquinone and azo dyes, we used shaking flask fermentation method to obtain the crude laccase solution. The results showed the Marasmius tricolor 310b can have a good decolorization effect on the three dyes bromocresol green, alizarin red and Congo red, and the benzomethane dye had the best decolorization. In addition, we found that the degradation degree of the crude enzyme solution of Marasmius tricolor 310b was related to the wavelength and reaction time, and with the reaction time increases, the degradation efficiency increased to different degrees. Our study revealed that the crude enzyme solution of the new strain Marasmius tricolor 310b had obvious decolorization effect on phenylmethane, anthraquinones and azo. This finding provided some theoretical support for updating the fungus resource bank with decolorization effect.
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https://doi.org/10.1016/j.bcab.2018.09.003
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